Analytical Data
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Gene name
Psmc1
- Application
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Alternative Names
P26s4; 26S proteasome AAA-ATPase subunit RPT2
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Species
Mouse
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Source
E. coli
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Tag
N-terminal His and Tag
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P62192
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Expression Region
1-440aa
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AA Sequence
MGQSQSGGHGPGGGKKDDKDKKKKYEPPVPTRVGKKKKKTKGPDAASKLPLVTP HTQCRLKLLKLERIKDYLLMEEEFIRNQEQMKPLEEKQEEERSKVDDLRGTPMS VGTLEEIIDDNHAIVSTSVGSEHYVSILSFVDKDLLEPGCSVLLNHKVHAVIGV LMDDTDPLVTVMKVEKAPQETYADIGGLDNQIQEIKESVELPLTHPEYYEEMGI KPPKGVILYGPPGTGKTLLAKAVANQTSATFLRVVGSELIQKYLGDGPKLVREL FRVAEEHAPSIVFIDEIDAIGTKRYDSNSGGEREIQRTMLELLNQLDGFDSRGD VKVIMATNRIETLDPALIRPGRIDRKIEFPLPDEKTKKRIFQIHTSRMTLADDV TLDDLIMAKDDLSGADIKAICTEAGLMALRERRMKVTNEDFKKSKENVLYKKQE GTPEGLYL
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Molecular Weight
52.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Psmc1, also known as Proteasome 26S subunit, is a vital component of the 26S proteasome, which plays a crucial role in the ubiquitin-proteasome pathway responsible for protein degradation within cells. This pathway maintains proteostasis, regulates various cellular processes, and is involved in the degradation of misfolded or damaged proteins, thereby preventing the accumulation of potentially toxic aggregates. Research into Psmc1 has gained significant attention due to its implications in several diseases, including cancer and neurodegenerative disorders, where proteasomal dysfunction is often observed. Psmc1 functions as part of the ATPase complex in the proteasome, facilitating the unfolding and translocation of substrates into the catalytic core for degradation. Given its essential role in cellular homeostasis, understanding the structure and function of Psmc1 through recombinant protein studies is pivotal. Such studies enhance our knowledge of proteasome regulation and its interactions with various substrates, paving the way for therapeutic interventions that target the proteasome system. Researchers are focusing on the production and characterization of recombinant Psmc1 to explore its mechanistic roles and potential as a target for drug development, particularly in conditions marked by proteasome inhibition or dysfunction.
