Analytical Data
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Gene name
FOXJ3
- Application
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Alternative Names
Forkhead box J3; Forkhead box protein J3; FOXJ3; FOXJ3_HUMAN; KIAA1041; MGC165036; MGC176686
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9UPW0
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Expression Region
1-622aa
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AA Sequence
MGLYGQACPSVTSLRMTSELESSLTSMDWLPQLTMRAAIQKSDATQNAHGTGISKKNALLDPNTTLDQEEVQQHKDGKPPYSYASLITFAINSSPKKKMTLSEIYQWICDNFPYYREAGSGWKNSIRHNLSLNKCFLKVPRSKDDPGKGSYWAIDTNPKEDVLPTRPKKRARSVERASTPYSIDSDSLGMECIISGSASPTLAINTVTNKVTLYNTDQDGSDSPRSSLNNSLSDQSLASVNLNSVGSVHSYTPVTSHPESVSQSLTPQQQPQYNLPERDKQLLFSEYNFEDLSASFRSLYKSVFEQSLSQQGLMNIPSESSQQSHTSCTYQHSPSSTVSTHPHSNQSSLSNSHGSGLNTTGSNSVAQVSLSHPQMHTQPSPHPPHRPHGLPQHPQRSPHPAPHPQQHSQLQSPHPQHPSPHQHIQHHPNHQHQTLTHQAPPPPQQVSCNSGVSNDWYATLDMLKESCRIASSVNWSDVDLSQFQGLMESMRQADLKNWSLDQVQFADLCSSLNQFFTQTGLIHSQSNVQQNVCHGAMHPTKPSQHIGTGNLYIDSRQNLPPSVMPPPGYPHIPQALSTPGTTMAGHHRAMNQQHMMPSQAFQMRRSLPPDDIQDDFDWDSIV
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Molecular Weight
68.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
FOXJ3, a member of the Forkhead box (FOX) protein family, has garnered significant attention in the field of molecular biology due to its critical role in the regulation of cilia formation and function. Cilia are microtubule-based organelles that play essential roles in various biological processes, including cell signaling, development, and the maintenance of tissue homeostasis. Dysregulation of ciliary function is implicated in a range of diseases, collectively known as ciliopathies, which can affect multiple organ systems. FOXJ3 is particularly important in the differentiation of motile cilia and is known to regulate genes involved in ciliary assembly and function. Research indicates that FOXJ3 is essential for the development of ciliated tissues, with its expression levels influencing cilia length and density. Moreover, studies have shown that FOXJ3 may interact with various signaling pathways that govern cilia biogenesis, highlighting its potential as a therapeutic target for conditions related to ciliary dysfunction. As such, the recombinant expression and characterization of FOXJ3 protein not only advance our understanding of ciliary biology but also pave the way for new insights into treating ciliopathies and related disorders.
