Analytical Data
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Gene name
HIST1H2AC
- Application
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Alternative Names
HIST1H2AC；H2AFL；HIST1H2AC；Histone H2A type 1-C
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q93077
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Expression Region
2-130aa
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AA Sequence
SGRGKQGGK ARAKAKSRSS RAGLQFPVGR VHRLLRKGNY AERVGAGAPV YLAAVLEYLT AEILELAGNA ARDNKKTRII PRHLQLAIRN DEELNKLLGR VTIAQGGVLP NIQAVLLPKK TESHHKAKGK
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HIST1H2AC is a member of the histone H2A family, which plays a crucial role in the packaging and regulation of DNA in eukaryotic cells. As a core component of the nucleosome, HIST1H2AC contributes to the structural integrity of chromatin and has been implicated in various cellular processes, including gene expression, DNA repair, and replication. Recent studies have highlighted the importance of post-translational modifications of histone proteins, such as acetylation and methylation, in the modulation of chromatin dynamics and gene accessibility. The histone variant HIST1H2AC, in particular, has garnered attention for its potential role in cancer biology, with aberrant expression patterns associated with tumorigenesis and metastasis. Research into the recombinant expression of HIST1H2AC allows for a deeper understanding of its functional implications and interactions within the chromatin context. By utilizing techniques such as recombinant DNA technology and protein purification, scientists can generate and study the functional characteristics of HIST1H2AC in vitro. Understanding the mechanistic pathways and regulatory networks involving this histone variant could provide valuable insights into epigenetic regulation and open avenues for therapeutic interventions in diseases associated with chromatin dysregulation. This line of research not only enhances our comprehension of fundamental biological processes but also expands the potential for utilizing histone modifications as biomarkers or targets for targeted therapies in oncology.
