Analytical Data
-
Gene name
ATG7
- Application
-
Alternative Names
ATG7；APG7L；Ubiquitin-like modifier-activating enzyme ATG7
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
O95352
-
Expression Region
2-703aa
-
AA Sequence
AAATGDPGL SKLQFAPFSS ALDVGFWHEL TQKKLNEYRL DEAPKDIKGY YYNGDSAGLP ARLTLEFSAF DMSAPTPARC CPAIGTLYNT NTLESFKTAD KKLLLEQAAN EIWESIKSGT ALENPVLLNK FLLLTFADLK KYHFYYWFCY PALCLPESLP LIQGPVGLDQ RFSLKQIEAL ECAYDNLCQT EGVTALPYFL IKYDENMVLV SLLKHYSDFF QGQRTKITIG VYDPCNLAQY PGWPLRNFLV LAAHRWSSSF QSVEVVCFRD RTMQGARDVA HSIIFEVKLP EMAFSPDCPK AVGWEKNQKG GMGPRMVNLS ECMDPKRLAE SSVDLNLKLM CWRLVPTLDL DKVVSVKCLL LGAGTLGCNV ARTLMGWGVR HITFVDNAKI SYSNPVRQPL YEFEDCLGGG KPKALAAADR LQKIFPGVNA RGFNMSIPMP GHPVNFSSVT LEQARRDVEQ LEQLIESHDV VFLLMDTRES RWLPAVIAAS KRKLVINAAL GFDTFVVMRH GLKKPKQQGA GDLCPNHPVA SADLLGSSLF ANIPGYKLGC YFCNDVVAPG DSTRDRTLDQ QCTVSRPGLA VIAGALAVEL MVSVLQHPEG GYAIASSSDD RMNEPPTSLG LVPHQIRGFL SRFDNVLPVS LAFDKCTACS SKVLDQYERE GFNFLAKVFN SSHSFLEDLT GLTLLHQETQ AAEIWDMSDD ETI
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ATG7, an essential gene in the autophagy process, plays a crucial role in the cellular degradation and recycling of proteins and organelles, thereby maintaining cellular homeostasis and responding to stress. It encodes a ubiquitin-like enzyme that activates autophagy by facilitating the conjugation of LC3 to phosphatidylethanolamine, a process vital for autophagosome formation. Research into recombinant ATG7 protein has gained traction due to its implications in various pathological conditions, including neurodegenerative diseases, cancer, and infections. Many of these diseases are characterized by impaired autophagy, leading to cellular dysfunction. Thus, understanding ATG7's function through recombinant studies can provide insights into its mechanistic role in autophagy and its contribution to disease processes. Moreover, recombinant ATG7 provides a valuable tool for exploring the autophagy pathway's dynamics in vitro and in vivo, enabling researchers to elucidate the molecular mechanisms underlying autophagosome formation and the impact of autophagy modulation. With advances in biotechnological methods, such as CRISPR and protein expression systems, studies focusing on recombinant ATG7 have the potential to identify novel therapeutic targets and strategies for enhancing autophagic activity, thereby offering promising avenues for treating various diseases linked to autophagy dysregulation. Through these investigations, scientists aim to bridge the gap between basic research and clinical applications, paving the way for innovative approaches to combat diseases where autophagy plays a critical role.
