Analytical Data
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Gene name
USF2
- Application
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Alternative Names
bHLHb12; Class B basic helix-loop-helix Protein 12; FIP; FOS interacting Protein; FOS-interacting Protein; Major late transcription factor 2; Upstream stimulatory factor 2; Upstream transcription factor 2; USF 2; Usf2; USF2_HUMAN
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q15853
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Expression Region
1-346 aa
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AA Sequence
MDMLDPGLDP AASATAAAAA SHDKGPEAEE GVELQEGGDG PGAEEQTAVA ITSVQQAAFG DHNIQYQFRT ETNGGQVTYR VVQVTDGQLD GQGDTAGAVS VVSTAAFAGG QQAVTQVGVD GAAQRPGPAA ASVPPGPAAP FPLAVIQNPF SNGGSPAAEA VSGEARFAYF PASSVGDTTA VSVQTTDQSL QAGGQFYVMM TPQDVLQTGT QRTIAPRTHP YSPKIDGTRT PRDERRRAQH NEVERRRRDK INNWIVQLSK IIPDCNADNS KTGASKGGIL SKACDYIREL RQTNQRMQET FKEAERLQMD NELLRQQIEE LKNENALLRA QLQQHNLEMV GEGTRQ
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Molecular Weight
36.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
USF2 (Upstream Stimulatory Factor 2) is a transcription factor that plays a critical role in regulating gene expression related to various biological processes, including cell growth, differentiation, and metabolism. Researchers have become increasingly interested in USF2 due to its implications in essential physiological functions and pathologies, including its association with cancer and metabolic disorders. The understanding of USF2's mechanisms has been hampered by a lack of purified recombinant protein, which is essential for in-depth biochemical analysis and functional studies. Therefore, the development of a reliable expression system for USF2 recombinant protein has become a priority. Advances in recombinant DNA technology and protein expression systems, such as bacterial, yeast, and mammalian cell lines, have allowed for the production of USF2 in sufficient quantities for characterization and functional assays. Investigating the structure, DNA-binding properties, and interaction with other cellular factors of USF2 will enhance our understanding of its role in health and disease. Furthermore, elucidating the signaling pathways involving USF2 could provide insights into targetable therapeutic strategies for conditions linked to its dysregulation. Researchers are focused on optimizing expression and purification protocols to obtain functional USF2 that can be used in structural biology studies, protein-protein interaction assays, and high-throughput screening for potential modulators of USF2 activity. Ultimately, the comprehensive study of USF2 recombinant protein aims to open new avenues for understanding its biological significance and therapeutic potential.
