Analytical Data
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Gene name
ATG16L1
- Application
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Alternative Names
ATG16L1；APG16L；Autophagy-related Protein 16-1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q676U5
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Expression Region
85-607aa
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AA Sequence
MAQLRIKHQEELTELHKKRGELAQLVIDLNNQMQRKDREMQMNEAKIAEC LQTISDLETECLDLRTKLCDLERANQTLKDEYDALQITFTALEGKLRKTT EENQELVTRWMAEKAQEANRLNAENEKDSRRRQARLQKELAEAAKEPLPV EQDDDIEVIVDETSDHTEETSPVRAISRAATKRLSQPAGGLLDSITNIFG RRSVSSFPVPQDNVDTHPGSGKEVRVPATALCVFDAHDGEVNAVQFSPGS RLLATGGMDRRVKLWEVFGEKCEFKGSLSGSNAGITSIEFDSAGSYLLAA SNDFASRIWTVDDYRLRHTLTGHSGKVLSAKFLLDNARIVSGSHDRTLKL WDLRSKVCIKTVFAGSSCNDIVCTEQCVMSGHFDKKIRFWDIRSESIVRE MELLGKITALDLNPERTELLSCSRDDLLKVIDLRTNAIKQTFSAPGFKCG SDWTRVVFSPDGSYVAAGSAEGSLYIWSVLTGKVEKVLSKQHSSSINAVA WSPSGSHVVSVDKGCKAVLWAQY
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Molecular Weight
85 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ATG16L1 is a key protein involved in the autophagy process, which is the body's way of cleaning out damaged cells and regenerating newer, healthier cells. Research has increasingly focused on ATG16L1 due to its association with various autoimmune diseases, particularly Crohn's disease, highlighting its crucial role in the immune response and inflammation. Mutations or dysregulation of ATG16L1 are linked to impaired autophagy, leading to the accumulation of bacteria and subsequent inflammatory responses in the gut. Understanding the structural and functional aspects of ATG16L1, especially through the study of recombinant proteins, allows researchers to investigate the molecular mechanisms underlying its role in autophagy. Such studies can elucidate how variations in ATG16L1 influence disease susceptibility, and pave the way for the development of therapeutic strategies aimed at modulating autophagy in related conditions. Consequently, the recombinant expression of ATG16L1 can facilitate detailed characterization and provide insights into its interactions with other cellular components, advancing the field of medical research focused on autophagy and immune dysregulation.
