Analytical Data
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基因名
FKRP
- Application
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别名
Fkrp; FKRP_HUMAN; FLJ12576; Fukutin related protein; Fukutin-related protein; LGMD2I; MDC1C; MGC2991
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9H9S5
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表达区间
396-494aa
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氨基酸序列
KAVEGDFFRVQYSESNHLHVDLWPFYPRNGVMTKDTWLDHRQDVEFPEHFLQPLVPLPFAGFVAQAPNNYRRFLELKFGPGVIENPQYPNPALLSLTGS
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分子量
36.63 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
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Protein Description
FKRP (Fukutin-related protein) is a glycosyltransferase implicated in the post-translational modification of dystroglycan, a protein critical for muscle integrity and function. Mutations in the FKRP gene are linked to a spectrum of muscle dystrophies, including limb-girdle muscular dystrophy 2I (LGMD2I) and congenital muscular dystrophy (CMD). The severity of these disorders often correlates with specific FKRP mutations, highlighting the need for a deeper understanding of FKRP's molecular function and its role in protein glycosylation pathways. Research into FKRP recombinant proteins has gained momentum to elucidate the structure-function relationships of the protein and to develop potential therapeutic strategies. By producing and characterizing FKRP in a controlled environment, scientists can study its enzymatic activity, substrate specificity, and interaction with other cellular components. Furthermore, recombinant FKRP offers a platform for high-throughput screening of small molecules that may enhance its function or compensate for the loss of activity due to mutations. Overall, the study of FKRP and its recombinant forms is crucial for advancing our knowledge of muscular dystrophies and developing targeted therapies that could mitigate the effects of FKRP-related disorders.
