Analytical Data
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Gene name
UBE2U
- Application
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Alternative Names
RP4-636O23.1; UBE2U; UBE2U_HUMAN; Ubiquitin carrier Protein U; Ubiquitin conjugating enzyme E2U (putative); Ubiquitin-conjugating enzyme E2 U; Ubiquitin-Protein ligase U
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q5VVX9
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Expression Region
1-321 aa
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AA Sequence
MHGRAYLLLH RDFCDLKENN YKGITAKPVS EDMMEWEVEI EGLQNSVWQG LVFQLTIHFT SEYNYAPPVV KFITIPFHPN VDPHTGQPCI DFLDNPEKWN TNYTLSSILL ALQVMLSNPV LENPVNLEAA RILVKDESLY RTILRLFNRP LQMKDDSQEL PKDPRKCIRP IKTTSFSDYY QTWSRIATSK ATEYYRTPLL KVPNFIGQYY KWKKMDLQHQ KEWNLKYSVI KCWLARKRMP HEVTHSMEEI KLCPTLIPTT DEIFLESPTA INSITDIYET EEEGWKSDTS LYENDTDEPR EEEVEDLISW TNTLNTNTSE D
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Molecular Weight
37.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
UBE2U, a member of the ubiquitin-conjugating enzyme family, plays a pivotal role in the ubiquitin-proteasome system, which regulates various cellular processes, including protein degradation, DNA repair, and cell cycle progression. This enzyme is particularly noteworthy due to its unique structure and distinct substrate specificity, which differentiates it from other E2 enzymes. Research has shown that UBE2U is involved in the modification of proteins that regulate key cellular functions, and its dysregulation has been linked to various diseases, including cancer and neurodegenerative disorders. Understanding the molecular mechanisms underlying UBE2U's function can provide insights into its potential as a therapeutic target for modulating ubiquitin-mediated pathways. Furthermore, the development of recombinant UBE2U proteins enables detailed biochemical and biophysical studies, allowing researchers to elucidate its interactions with E3 ligases and substrate proteins. This research could pave the way for novel therapeutic strategies that exploit the ubiquitin-proteasome system to treat diseases characterized by proteostasis imbalance. As such, UBE2U represents a critical focal point in the investigation of ubiquitin biology, with significant implications for understanding cellular homeostasis and developing targeted interventions.
