Analytical Data
-
Gene name
UBE2E2
- Application
-
Alternative Names
FLJ25157; UB2E2_HUMAN; UBC4/5 homolog yeast; UBCH 8; UbcH8; Ube2e2; Ubiquitin carrier Protein E2; Ubiquitin conjugating enzyme E2 E2; Ubiquitin conjugating enzyme E2E 2 (homologous to yeast UBC4/5); Ubiquitin conjugating enzyme E2E 2 (UBC4/5 homolog yeast); Ubiquitin conjugating enzyme E2E 2; Ubiquitin Protein ligase E2; Ubiquitin-conjugating enzyme E2 E2; Ubiquitin-Protein ligase E2
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q96LR5
-
Expression Region
2-201 aa
-
AA Sequence
STEAQRVDD SPSTSGGSSD GDQRESVQQE PEREQVQPKK KEGKISSKTA AKLSTSAKRI QKELAEITLD PPPNCSAGPK GDNIYEWRST ILGPPGSVYE GGVFFLDITF SPDYPFKPPK VTFRTRIYHC NINSQGVICL DILKDNWSPA LTISKVLLSI CSLLTDCNPA DPLVGSIATQ YMTNRAEHDR MARQWTKRYA T
-
Molecular Weight
22.2 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
UBE2E2, a member of the E2 ubiquitin-conjugating enzyme family, plays a crucial role in the ubiquitin-proteasome system, which is essential for maintaining cellular homeostasis by regulating protein degradation, signaling, and various cellular processes. The dysregulation of UBE2E2 has been associated with several diseases, including cancer, where it can influence tumor progression and response to therapy. Recent studies have highlighted its functional significance in mediating the ubiquitination of specific substrates, thereby modulating their stability and activity. Research into UBE2E2 has gained momentum as scientists aim to unravel its precise mechanisms of action, interactions with E3 ligases, and the impact of mutations or alterations in its expression levels. Understanding UBE2E2 at the molecular level could offer insights into its potential as a therapeutic target, with the development of small molecules or inhibitors that could manipulate its function to treat diseases linked to ubiquitin-mediated regulation. Furthermore, ongoing investigations into the structural biology of UBE2E2, including its catalytic activity and substrate specificity, are providing new opportunities for drug design and discovery, aiming for innovative strategies to combat diseases rooted in protein misregulation.
