Analytical Data
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基因名
UBE1C
- Application
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别名
DKFZp566J164; EC 6.3.2.; hUba3; MGC22384; NEDD8 activating enzyme E1 catalytic subunit; NEDD8 activating enzyme E1C; Nedd8 activating enzyme hUba3; NEDD8-activating enzyme E1 catalytic subunit; NEDD8-activating enzyme E1C; uba3; UBA3 ubiquitin activating enzyme E1 homolog; UBA3_HUMAN; UBE1C; Ubiquitin activating enzyme 3; Ubiquitin activating enzyme E1C; Ubiquitin-activating enzyme 3; Ubiquitin-activating enzyme E1C; Ubiquitin-like modifier-activating enzyme 3
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8TBC4
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表达区间
2-463 aa
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氨基酸序列
ADGEEPEKK RRRIEELLAE KMAVDGGCGD TGDWEGRWNH VKKFLERSGP FTHPDFEPST ESLQFLLDTC KVLVIGAGGL GCELLKNLAL SGFRQIHVID MDTIDVSNLN RQFLFRPKDI GRPKAEVAAE FLNDRVPNCN VVPHFNKIQD FNDTFYRQFH IIVCGLDSII ARRWINGMLI SLLNYEDGVL DPSSIVPLID GGTEGFKGNA RVILPGMTAC IECTLELYPP QVNFPMCTIA SMPRLPEHCI EYVRMLQWPK EQPFGEGVPL DGDDPEHIQW IFQKSLERAS QYNIRGVTYR LTQGVVKRII PAVASTNAVI AAVCATEVFK IATSAYIPLN NYLVFNDVDG LYTYTFEAER KENCPACSQL PQNIQFSPSA KLQEVLDYLT NSASLQMKSP AITATLEGKN RTLYLQSVTS IEERTRPNLS KTLKELGLVD GQELAVADVT TPQTVLFKLH FTS
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分子量
51.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
UBE1C (Ubiquitin-activating enzyme 1C) is a crucial enzyme involved in the ubiquitin-proteasome system, which regulates protein degradation and homeostasis within cells. This enzyme plays a significant role in tagging proteins for degradation via the attachment of ubiquitin molecules, thus influencing various cellular processes such as cell cycle regulation, DNA repair, and signal transduction. Research on UBE1C has gained prominence due to its implications in cancer biology, as altered expression and activity of ubiquitin-conjugating enzymes like UBE1C can lead to uncontrolled cell proliferation and tumor development. Additionally, UBE1C has been identified as a potential target for therapeutic intervention; its inhibitors might serve as novel anti-cancer agents. Understanding the structure-function relationship of the UBE1C protein, along with its interaction with other proteins in the ubiquitin pathway, can provide insights into its role in disease mechanisms and open avenues for drug development. As a result, the recombinant expression and characterization of UBE1C are of significant interest to researchers, facilitating the study of its enzymatic activity and potential as a biomarker or drug target in various malignancies.
