Analytical Data
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Gene name
NRF1
- Application
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Alternative Names
NRF1；Nuclear respiratory factor 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96AN2
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Expression Region
1-522aa
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AA Sequence
MEEHGVTQTEHMATIEAHAVAQQVQQVHVATYTEHSMLSADEDSPSSPED TSYDDSDILNSTAADEVTAHLAAAGPVGMAAAAAVATGKKRKRPHVFESN PSIRKRQQTRLLRKLRATLDEYTTRVGQQAIVLCISPSKPNPVFKVFGAA PLENVVRKYKSMILEDLESALAEHAPAPQEVNSELPPLTIDGIPVSVDKM TQAQLRAFIPEMLKYSTGRGKPGWGKESCKPIWWPEDIPWANVRSDVRTE EQKQRVSWTQALRTIVKNCYKQHGREDLLYAFEDQQTQTQATATHSIAHL VPSQTVVQTFSNPDGTVSLIQVGTGATVATLADASELPTTVTVAQVNYSA VADGEVEQNWATLQGGEMTIQTTQASEATQAVASLAEAAVAASQEMQQGA TVTMALNSEAAAHAVATLAEATLQGGGQIVLSGETAAAVGALTGVQDANG LFMADRAGRKWILTDKATGLVQIPVSMYQTVVTSLAQGNGPVQVAMAPVT TRISDSAVTMDGQAVEVVTLEQ
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Molecular Weight
82 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Nuclear respiratory factor 1 (NRF1) is a crucial transcription factor that plays a significant role in the regulation of mitochondrial biogenesis and cellular respiration. NRF1 is essential for the transcriptional activation of genes necessary for the mitochondrial oxidative phosphorylation system, which is vital for energy production in eukaryotic cells. Dysregulation of NRF1 has been implicated in various metabolic disorders, aging-related diseases, and neurodegenerative conditions, highlighting its potential as a therapeutic target. The recombinant expression of NRF1 in heterologous systems has become a critical area of study, enabling researchers to investigate its structure-function relationships, post-translational modifications, and interactions with other cellular pathways. By producing NRF1 as a recombinant protein, scientists can explore its specific roles in mitochondrial function and energy metabolism, paving the way for novel insights into metabolic regulation and potential interventions for diseases associated with mitochondrial dysfunction. The findings from these studies could have significant implications for understanding the molecular mechanisms underlying various human diseases and for developing therapeutic strategies aimed at enhancing mitochondrial health and function.
