Analytical Data
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基因名
TWIST1
- Application
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别名
ACS3; B-HLH DNA binding Protein; bHLHa38; BPES2; BPES3; Class A basic helix-loop-helix Protein 38; CRS; CRS1; CSO; H-twist; OTTHUMP00000116043; SCS; Twist basic helix loop helix transcription factor 1; Twist family bHLH transcription factor 1; Twist homolog 1 (Drosophila); Twist homolog 1; TWIST homolog of drosophila; Twist related Protein 1; Twist-related Protein 1; TWIST1; TWST1_HUMAN
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q15672
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表达区间
1-202 aa
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氨基酸序列
MMQDVSSSPV SPADDSLSNS EEEPDRQQPP SGKRGGRKRR SSRRSAGGGA GPGGAAGGGV GGGDEPGSPA QGKRGKKSAG CGGGGGAGGG GGSSSGGGSP QSYEELQTQR VMANVRERQR TQSLNEAFAA LRKIIPTLPS DKLSKIQTLK LAARYIDFLY QVLQSDELDS KMASCSYVAH ERLSYAFSVW RMEGAWSMSA SH
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分子量
20.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TWIST1 is a vital transcription factor belonging to the basic helix-loop-helix (bHLH) family, playing a crucial role in various developmental processes, including embryogenesis, lineage commitment, and cellular differentiation. Its function in regulating mesodermal development and neural crest formation makes it a key player in morphogenetic events. Additionally, TWIST1 is implicated in the epithelial-mesenchymal transition (EMT), a process critical for cancer progression and metastasis. Previous studies have identified dysregulation of TWIST1 expression in various cancers, such as breast, gastric, and lung, linking its overexpression to enhanced migratory and invasive capabilities of tumor cells. Given the significance of TWIST1 in both developmental biology and cancer, there is a growing interest in characterizing TWIST1 as a recombinant protein for functional studies. Such research aims to elucidate the molecular mechanisms underlying its role in EMT, identify potential downstream targets, and investigate its interactions with other cellular pathways. Producing TWIST1 as a recombinant protein also opens avenues for developing therapeutic strategies targeting TWIST1-related pathways in cancer. Through this research, we hope to provide deeper insights into the biological functions of TWIST1 and its potential as a biomarker or therapeutic target in oncological settings.
