Analytical Data
-
基因名
FBXW12
- Application
-
别名
FBXW12; FBW12; FBXO12; FBXO35F-box/WD repeat-containing protein 12; F-box and WD-40 domain-containing protein 12; F-box only protein 35
-
种属
Human
-
表达系统
E. coli
-
标签
GST-tag at N-terminal
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q6X9E4
-
表达区间
1-445aa
-
氨基酸序列
MSPTHQIQDPKHWNRIADSDYLWRSLSLQRWDCSNFTNQHLGTHTWKQFFLHQRRKELRLALAQPHNFIYKVTKNIAFETELAYLSGNRLTVDEQEKSIICSVSPKQELCAWDVQEGTMIWSSPVQEFHFSNLVTLPQMHLAITMDRKKTIKVWNCQDRDALAVLPMPQPCYCMEAYLTKDGPFLMVGDAAGDIYTFTLPGLRDVSKVTAFQYGIVLLHCSPDKKWVFACGTYSRTLPQVFLTESLLRPSEGSDPLSTLLPHKLCASACWTPKVKNRITLMSQSSTGKKTEFITFDLTTKKTGGQTVIQAYEIASFQVAAHLKCPIWMGASDGYMIVFTSGPYLLLFSITGFLLQRFEDHQAAINNFWVDPCYVLTTSENSVHVYMWEEGGRHPYLRSCCHLENTWHDHTTDSCISSVMCDNASIVLRVRKVSDSSILVMYSLNT
-
分子量
77.2 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
FBXW12 is a member of the F-box protein family, known for its role in targeting proteins for ubiquitination and subsequent degradation via the proteasome. This class of proteins plays a critical role in various cellular processes, including cell cycle regulation, signal transduction, and apoptosis. Research into FBXW12 has gained momentum due to its involvement in the regulation of key oncogenic pathways, particularly its interplay with the tumor suppressor p53 and other cell cycle regulators. Abnormal expression or mutation of FBXW12 has been implicated in several cancers, suggesting its potential as a biomarker for tumor progression and as a target for therapeutic intervention. Furthermore, understanding the mechanisms by which FBXW12 modulates protein stability and cell signaling can provide insights into novel cancer treatment strategies. The study of FBXW12's biological functions and regulatory mechanisms continues to be a significant focus within the fields of molecular biology and oncology, highlighting its importance in the broader context of cell regulation and disease pathology.
