Analytical Data
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Gene name
FBXW12
- Application
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Alternative Names
FBXW12; FBW12; FBXO12; FBXO35F-box/WD repeat-containing protein 12; F-box and WD-40 domain-containing protein 12; F-box only protein 35
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q6X9E4
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Expression Region
1-445aa
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AA Sequence
MSPTHQIQDPKHWNRIADSDYLWRSLSLQRWDCSNFTNQHLGTHTWKQFFLHQRRKELRLALAQPHNFIYKVTKNIAFETELAYLSGNRLTVDEQEKSIICSVSPKQELCAWDVQEGTMIWSSPVQEFHFSNLVTLPQMHLAITMDRKKTIKVWNCQDRDALAVLPMPQPCYCMEAYLTKDGPFLMVGDAAGDIYTFTLPGLRDVSKVTAFQYGIVLLHCSPDKKWVFACGTYSRTLPQVFLTESLLRPSEGSDPLSTLLPHKLCASACWTPKVKNRITLMSQSSTGKKTEFITFDLTTKKTGGQTVIQAYEIASFQVAAHLKCPIWMGASDGYMIVFTSGPYLLLFSITGFLLQRFEDHQAAINNFWVDPCYVLTTSENSVHVYMWEEGGRHPYLRSCCHLENTWHDHTTDSCISSVMCDNASIVLRVRKVSDSSILVMYSLNT
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Molecular Weight
77.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
FBXW12 is a member of the F-box protein family, known for its role in targeting proteins for ubiquitination and subsequent degradation via the proteasome. This class of proteins plays a critical role in various cellular processes, including cell cycle regulation, signal transduction, and apoptosis. Research into FBXW12 has gained momentum due to its involvement in the regulation of key oncogenic pathways, particularly its interplay with the tumor suppressor p53 and other cell cycle regulators. Abnormal expression or mutation of FBXW12 has been implicated in several cancers, suggesting its potential as a biomarker for tumor progression and as a target for therapeutic intervention. Furthermore, understanding the mechanisms by which FBXW12 modulates protein stability and cell signaling can provide insights into novel cancer treatment strategies. The study of FBXW12's biological functions and regulatory mechanisms continues to be a significant focus within the fields of molecular biology and oncology, highlighting its importance in the broader context of cell regulation and disease pathology.
