Analytical Data
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Gene name
TSSK1B
- Application
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Alternative Names
TSSK1B; SPOGA1; SPOGA4; STK22A; STK22D; TSSK1; FKSG81; Testis-specific serine/threonine-Protein kinase 1; TSK-1; TSK1; TSSK-1; Testis-specific kinase 1; EC 2.7.11.1; Serine/threonine-Protein kinase 22A
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BXA7
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Expression Region
1-367 aa
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AA Sequence
MDDAAVLKRR GYLLGINLGE GSYAKVKSAY SERLKFNVAI KIIDRKKAPA DFLEKFLPRE IEILAMLNHC SIIKTYEIFE TSHGKVYIVM ELAVQGDLLE LIKTRGALHE DEARKKFHQL SLAIKYCHDL DVVHRDLKCD NLLLDKDFNI KLSDFSFSKR CLRDDSGRMA LSKTFCGSPA YAAPEVLQGI PYQPKVYDIW SLGVILYIMV CGSMPYDDSN IKKMLRIQKE HRVNFPRSKH LTGECKDLIY HMLQPDVNRR LHIDEILSHC WMQPKARGSP SVAINKEGES SRGTEPLWTP EPGSDKKSAT KLEPEGEAQP QAQPETKPEG TAMQMSRQSE ILGFPSKPST METEEGPPQQ PPETRAQ
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Molecular Weight
41.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TSSK1B (Testis-Specific Serine Kinase 1B) is a member of the TSSK family, which plays a crucial role in male fertility and spermatogenesis. Research into TSSK1B has gained momentum due to its potential implications in male reproductive health, as it is specifically expressed in testicular tissues. Previous studies have indicated that TSSK1B is involved in the regulation of sperm motility and is crucial for the maturation of spermatozoa. Disruptions in TSSK1B function or expression levels have been associated with various male infertility issues, making it a significant target for understanding the molecular mechanisms underlying spermatogenic defects. Additionally, the characterization of TSSK1B as a recombinant protein has provided insights into its biological functions, substrate specificity, and interaction with other proteins involved in sperm development. The production and study of TSSK1B recombinant protein enable researchers to explore its kinase activity and regulatory pathways in controlled experimental settings. These investigations could pave the way for novel therapeutic strategies to address male infertility and further our understanding of the mechanistic roles of serine kinases in reproductive biology.
