Analytical Data
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Gene name
PIKFYVE
- Application
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Alternative Names
PIKFYVE；KIAA0981；PIP5K3；1-phosphatidylinositol 3-phosphate 5-kinase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9Y2I7
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Expression Region
1493-2098aa
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AA Sequence
KKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMD ASPRNISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGG PPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPF PFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQ WNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPTKKASG MLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGVEPQD EVDGGDTQKKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSE EDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAP HYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFY GRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRS HSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFT WDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWT GLGLNC
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Molecular Weight
93 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PIKFYVE is a phosphoinositide kinase that plays a critical role in cellular processes such as membrane trafficking, endosomal dynamics, and signal transduction. It specifically phosphorylates phosphatidylinositol-5-phosphate (PI5P) to generate phosphatidylinositol-5, 7-bisphosphate (PI(5,7)P2), which is essential for various cellular functions, including vesicle formation and intracellular signaling pathways. Dysregulation of PIKFYVE has been implicated in several diseases, including neurodegenerative conditions and cancer, highlighting its importance as a therapeutic target. Research on recombinant PIKFYVE protein focuses on understanding its structure, catalytic mechanism, and functional interactions with other cellular components. By studying recombinant PIKFYVE, scientists aim to elucidate the molecular underpinnings of its role in cellular pathways and its potential implications in disease states. Furthermore, the development of specific inhibitors or modulators of PIKFYVE activity could lead to novel therapeutic strategies for tackling diseases associated with PIKFYVE dysfunction. This research presents a significant opportunity to advance our understanding of lipid signaling and membrane biology, ultimately contributing to the development of targeted treatments for related disorders.
