Analytical Data
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基因名
TRIO
- Application
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别名
ARHGEF23; MEBAS; MRD44; PTPRF-interacting Protein; tgat; trio; Trio Rho guanine nucleotide exchange factor; TRIO_HUMAN; Triple functional domain (PTPRF interacting); Triple functional domain Protein
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O75962
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表达区间
1-372 aa
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氨基酸序列
MTPDLLNFKKGWMSILDEPGEPPSPSLTTTSTSQWKKHWFVLTDSSLKYYRDSTAEEADELDGEIDLRSCTDVTEYAVQRNYGFQIHTKDAVYTLSAMTSGIRRNWIEALRKTVRPTSAPDVTKLSDSNKENALHSYSTQKGPLKAGEQRAGSEVISRGGPRKADGQRQALDYVELSPLTQASPQRARTPARTPDRLAKQEELERDLAQRSEERRKWFEATDSRTPEVPAGEGPRRGLGAPLTEDQQNRLSEEIEKKWQELEKLPLRENKRVPLTALLNQSRGERRGPPSDGHEALEKEVQALRAQLEAWRLQGEAPQSALRSQEDGHIPPGYISQLVGVITVPVLQTRPLSSERLCDLPKVTPPAGLKGGI
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分子量
67.32 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TRIO (Trio Rho Guanine Nucleotide Exchange Factor) is a multidomain protein that functions as a guanine nucleotide exchange factor, primarily involved in the regulation of small GTPases such as Rac1, RhoA, and Cdc42. These GTPases play crucial roles in various cellular processes, including cytoskeletal dynamics, cell migration, and neuronal development. TRIO is known to be implicated in several physiological processes, including those related to brain development and immune responses, and has been linked to certain cancers and neurodegenerative diseases due to its role in cell signaling pathways. Given its significance, TRIO has become a focus of research aimed at understanding its structural and functional characteristics. Researchers have employed recombinant protein techniques to produce TRIO, facilitating the investigation of its biochemical properties, interaction mechanisms with GTPases, and regulatory roles in signaling cascades. By studying TRIO in detail, scientists aim to elucidate its contributions to cellular behavior and pathogeneses linked to its dysfunction, opening pathways for potential therapeutic interventions targeting TRIO-related pathways in diseases.
