Cat: PAX2000-12180

Recombinant Human TRIM6 Protein,His

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Analytical Data

  • Gene name

    TRIM6

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    TRIM6; RNF89; Tripartite motif-containing Protein 6; EC 2.3.2.27; RING finger Protein 89; RING-type E3 ubiquitin transferase TRIM6

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q9C030

  • Expression Region

    1-488 aa

  • AA Sequence

    MTSPVLVDIR EEVTCPICLE LLTEPLSIDC GHSFCQACIT PNGRESVIGQ EGERSCPVCQ TSYQPGNLRP NRHLANIVRR LREVVLGPGK QLKAVLCADH GEKLQLFCQE DGKVICWLCE RSQEHRGHHT FLVEEVAQEY QEKFQESLKK LKNEEQEAEK LTAFIREKKT SWKNQMEPER CRIQTEFNQL RNILDRVEQR ELKKLEQEEK KGLRIIEEAE NDLVHQTQSL RELISDLERR CQGSTMELLQ DVSDVTERSE FWTLRKPEAL PTKLRSMFRA PDLKRMLRVC RELTDVQSYW VDVTLNPHTA NLNLVLAKNR RQVRFVGAKV SGPSCLEKHY DCSVLGSQHF SSGKHYWEVD VAKKTAWILG VCSNSLGPTF SFNHFAQNHS AYSRYQPQSG YWVIGLQHNH EYRAYEDSSP SLLLSMTVPP RRVGVFLDYE AGTVSFYNVT NHGFPIYTFS KYYFPTTLCP YFNPCNCVIP MTLRRPSS

  • Molecular Weight

    56.4 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

TRIM6, a member of the Tripartite Motif (TRIM) family of proteins, plays a significant role in the immune response and antiviral defense mechanisms. The TRIM family is characterized by a RING finger domain, two B-box domains, and a coiled-coil region, which are crucial for mediating various cellular functions, including ubiquitination, protein-protein interactions, and modulation of signaling pathways. Research on TRIM6 has revealed its involvement in the regulation of immune responses against viral infections, particularly through the enhancement of interferon signaling and direct antiviral activities. Studies have shown that TRIM6 can facilitate the degradation of viral proteins and modulate the production of pro-inflammatory cytokines, highlighting its potential as a therapeutic target for viral diseases. Furthermore, the dysregulation of TRIM6 has been linked to different pathological conditions, including autoimmune diseases and cancer, underscoring its importance in maintaining cellular homeostasis. Given its multifaceted roles in immune regulation, the recombinant expression and characterization of TRIM6 can provide valuable insights into its functional mechanisms and therapeutic applications, paving the way for innovative strategies in combating viral infections and associated diseases.

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