Analytical Data
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Gene name
PTBP1
- Application
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Alternative Names
PTBP1；PTB；Polypyrimidine tract-binding Protein 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P26599-3
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Expression Region
1-557aa
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AA Sequence
MDGIVPDIAVGTKRGSDELFSTCVTNGPFIMSSNSASAANGNDSKKFKGD SRSAGVPSRVIHIRKLPIDVTEGEVISLGLPFGKVTNLLMLKGKNQAFIE MNTEEAANTMVNYYTSVTPVLRGQPIYIQFSNHKELKTDSSPNQARAQAA LQAVNSVQSGNLALAASAAAVDAGMAMAGQSPVLRIIVENLFYPVTLDVL HQIFSKFGTVLKIITFTKNNQFQALLQYADPVSAQHAKLSLDGQNIYNAC CTLRIDFSKLTSLNVKYNNDKSRDYTRPDLPSGDSQPSLDQTMAAAFGAP GIISASPYAGAGFPPTFAIPQAAGLSVPNVHGALAPLAIPSAAAAAAAAG RIAIPGLAGAGNSVLLVSNLNPERVTPQSLFILFGVYGDVQRVKILFNKK ENALVQMADGNQAQLAMSHLNGHKLHGKPIRITLSKHQNVQLPREGQEDQ GLTKDYGNSPLHRFKKPGSKNFQNIFPPSATLHLSNIPPSVSEEDLKVLF SSNGGVVKGFKFFQKDRKMALIQMGSVEEAVQALIDLHNHDLGENHHLRV SFSKSTI
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Molecular Weight
87 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PTBP1 (Polypyrimidine Tract Binding Protein 1) is a significant RNA-binding protein that plays a vital role in various cellular processes, including alternative splicing, mRNA regulation, and gene expression. Its ability to bind to polypyrimidine tracts and other RNA elements allows PTBP1 to influence the stability, localization, and translation of target mRNAs. Research has indicated that PTBP1 is intricately involved in developmental processes and is implicated in several diseases, including cancer. The study of PTBP1 recombinant protein is crucial for understanding its functional mechanisms and interactions at the molecular level. By utilizing recombinant DNA technology, researchers can produce PTBP1 in vitro, facilitating detailed analysis of its RNA-binding properties, post-translational modifications, and the effects of specific mutations. This experimental approach also aids in exploring PTBP1's role in splicing regulation and its potential impact on disease pathogenesis. Given the protein's significance in cellular processes and disease, PTBP1 recombinant protein research is a promising area, offering insights that could lead to novel therapeutic strategies targeting its regulatory functions and interactions.
