Analytical Data
-
基因名
EVC
- Application
-
别名
EVCEllis-van Creveld syndrome protein; DWF-1
-
种属
Human
-
表达系统
E. coli
-
标签
GST-tag at N-terminal
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
P57679
-
表达区间
1-535aa
-
氨基酸序列
MARGGAACKSDARLLLGRDALRPAPALLAPAVLLGAALGLGLGLWLGCRAGRQRTRHQKDDTQNLLKNLESNAPTPSETGSPSRRRKREVQMSKDKEAVDECEPPSNSNITAFALKAKVIYPINQKFRPLADGSSNPSLHENLKQAVLPHQPVEASPSSSLGSLSQGEKDDCSSSSSVHSATSDDRFLSRTFLRVNAFPEVLACESVDVDLCIYSLHLKDLLHLDTALRQEKHMMFIQIFKMCLLDLLPKKKSDDELHQKILSKQEKDLEELEKGLQVKLSNTEMSGAGDSEYITLADVEKKEREYSEQLIDNMEAFWKQMANIQHFLVDQFKCSSSKARQLMMTLTERMIAAEGLLCDSQELQALDALERTMGRAHMAKVIEFLKLQVQEETRCRLAAISHGLELLAGEGKLSGRQKEELLTQQHKAFWQEAERFSREFVQRGKDLVKASLAHQVEGTAKLTLAQEEEQRSFLAEAQPTADPEKFLEAFHEVLERQRLMQCDLEEEENVRATEAVVALCQKAMRRHRDTGKEAV
-
分子量
86.4 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of EVC (Ellis-van Creveld) proteins has gained significant attention due to their critical role in skeletal dysplasia and developmental disorders. EVC is associated with a rare genetic condition known as Ellis-van Creveld syndrome, characterized by short stature, polydactyly, and cardiac malformations. Understanding the molecular mechanisms of EVC proteins is crucial for elucidating the pathogenesis of this syndrome and developing potential therapeutic strategies. Research into the EVC protein family has revealed its involvement in the primary cilia, which are sensory organelles crucial for cell signaling and development. Investigations aim to uncover how mutations in EVC genes disrupt normal protein function and lead to the clinical manifestations observed in affected individuals. Furthermore, insights into EVC proteins may contribute to broader knowledge regarding the regulation of the hedgehog signaling pathway, which is vital for embryonic development. The dynamic nature of EVC protein interactions, their localization, and post-translational modifications present a rich area for exploration, with implications for understanding similar genetic disorders. As research advances, there is potential for the identification of biomarkers for early diagnosis and the development of targeted therapies to improve the quality of life for individuals affected by EVC-related syndromes.
