Analytical Data
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Gene name
EVC
- Application
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Alternative Names
EVCEllis-van Creveld syndrome protein; DWF-1
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P57679
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Expression Region
1-535aa
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AA Sequence
MARGGAACKSDARLLLGRDALRPAPALLAPAVLLGAALGLGLGLWLGCRAGRQRTRHQKDDTQNLLKNLESNAPTPSETGSPSRRRKREVQMSKDKEAVDECEPPSNSNITAFALKAKVIYPINQKFRPLADGSSNPSLHENLKQAVLPHQPVEASPSSSLGSLSQGEKDDCSSSSSVHSATSDDRFLSRTFLRVNAFPEVLACESVDVDLCIYSLHLKDLLHLDTALRQEKHMMFIQIFKMCLLDLLPKKKSDDELHQKILSKQEKDLEELEKGLQVKLSNTEMSGAGDSEYITLADVEKKEREYSEQLIDNMEAFWKQMANIQHFLVDQFKCSSSKARQLMMTLTERMIAAEGLLCDSQELQALDALERTMGRAHMAKVIEFLKLQVQEETRCRLAAISHGLELLAGEGKLSGRQKEELLTQQHKAFWQEAERFSREFVQRGKDLVKASLAHQVEGTAKLTLAQEEEQRSFLAEAQPTADPEKFLEAFHEVLERQRLMQCDLEEEENVRATEAVVALCQKAMRRHRDTGKEAV
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Molecular Weight
86.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of EVC (Ellis-van Creveld) proteins has gained significant attention due to their critical role in skeletal dysplasia and developmental disorders. EVC is associated with a rare genetic condition known as Ellis-van Creveld syndrome, characterized by short stature, polydactyly, and cardiac malformations. Understanding the molecular mechanisms of EVC proteins is crucial for elucidating the pathogenesis of this syndrome and developing potential therapeutic strategies. Research into the EVC protein family has revealed its involvement in the primary cilia, which are sensory organelles crucial for cell signaling and development. Investigations aim to uncover how mutations in EVC genes disrupt normal protein function and lead to the clinical manifestations observed in affected individuals. Furthermore, insights into EVC proteins may contribute to broader knowledge regarding the regulation of the hedgehog signaling pathway, which is vital for embryonic development. The dynamic nature of EVC protein interactions, their localization, and post-translational modifications present a rich area for exploration, with implications for understanding similar genetic disorders. As research advances, there is potential for the identification of biomarkers for early diagnosis and the development of targeted therapies to improve the quality of life for individuals affected by EVC-related syndromes.
