Analytical Data
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Gene name
TMED4
- Application
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Alternative Names
Endoplasmic reticulum stress-response Protein 25; ERS25; GMP25iso; HNLF; p24 family Protein alpha-3; p24alpha3; Putative NF-kappa-B-activating Protein 156; Putative NFkB activating Protein HNLF; TMED4; TMED4_HUMAN; Transmembrane emp24 domain containing Protein 4; Transmembrane emp24 domain-containing Protein 4; Transmembrane emp24 Protein transport domain containing 4
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q7Z7H5
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Expression Region
1-227 aa
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AA Sequence
MAGVGAGPLRAMGRQALLLLALCATGAQGLYFHIGETEKRCFIEEIPDETMVIGNYRTQMWDKQKEVFLPSTPGLGMHVEVKDPDGKVVLSRQYGSEGRFTFTSHTPGDHQICLHSNSTRMALFAGGKLRVHLDIQVGEHANNYPEIAAKDKLTELQLRARQLLDQVEQIQKEQDYQRYREERFRLTSESTNQRVLWWSIAQTVILILTGIWQMRHLKSFFEAKKLV
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Molecular Weight
52.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
TMED4, also known as transmembrane emp20 domain-containing protein 4, is a member of the TMED family, which plays a crucial role in the transport and sorting of proteins within the secretory pathway. Research on TMED4 has gained significance due to its involvement in various cellular processes, including protein glycosylation and the regulation of endoplasmic reticulum-associated degradation (ERAD). Aberrant expression of TMED4 has been linked to several pathological conditions, including cancer and neurodegenerative disorders, highlighting its potential as a biomarker and therapeutic target. The recombinant expression of TMED4 in model systems facilitates the study of its structural and functional properties, advancing our understanding of its role in cellular homeostasis and the mechanisms underlying disease. Characterizing recombinant TMED4 can provide insights into its interaction with other proteins, elucidating its specific contributions to intracellular trafficking. Furthermore, the generation of recombinant TMED4 may enable the development of novel pharmacological interventions aimed at modulating its function, ultimately paving the way for innovative treatment strategies in diseases linked to protein misfolding and trafficking anomalies. These aspects underscore the relevance of TMED4 in both basic and applied biomedical research, making it a compelling subject for further investigation.
