Analytical Data
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基因名
APAF1
- Application
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别名
APAF1；Methylthioribulose-1-phosphate dehydratase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O14727-2
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表达区间
2-1237aa
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氨基酸序列
MHHHHHHDYKDDDDKDAKARNCLLQHREALEKDIKTSYIMDHMISDGFLT ISEEEKVRNEPTQQQRAAMLIKMILKKDNDSYVSFYNALLHEGYKDLAAL LHDGIPVVSSSSVRTVLCEGGVPQRPVVFVTRKKLVNAIQQKLSKLKGEP GWVTIHGMAGCGKSVLAAEAVRDHSLLEGCFPGGVHWVSVGKQDKSGLLM KLQNLCTRLDQDESFSQRLPLNIEEAKDRLRILMLRKHPRSLLILDDVWD SWVLKAFDSQCQILLTTRDKSVTDSVMGPKYVVPVESSLGKEKGLEILSL FVNMKKADLPEQAHSIIKECKGSPLVVSLIGALLRDFPNRWEYYLKQLQN KQFKRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPT KVLCILWDMETEEVEDILQEFVNKSLLFCDRNGKSFRYYLHDLQVDFLTE KNCSQLQDLHKKIITQFQRYHQPHTLSPDQEDCMYWYNFLAYHMASAKMH KELCALMFSLDWIKAKTELVGPAHLIHEFVEYRHILDEKDCAVSENFQEF LSLNGHLLGRQPFPNIVQLGLCEPETSEVYQQAKLQAKQEVDNGMLYLEW INKKNITNLSRLVVRPHTDAVYHACFSEDGQRIASCGADKTLQVFKAETG EKLLEIKAHEDEVLCCAFSTDDRFIATCSVDKKVKIWNSMTGELVHTYDE HSETQVNCCHFTNSSHHLLLATGSSDCFLKLWDLNQKECRNTMFGHTNSV NHCRFSPDDKLLASCSADGTLKLWDATSANERKSINVKQFFLNLEDPQED MEVIVKCCSWSADGARIMVAAKNKIFLFDIHTSGLLGEIHTGHHSTIQYC DFSPQNHLAVVALSQYCVELWNTDSRSKVADCRGHLSWVHGVMFSPDGSS FLTSSDDQTIRLWETKKVCKNSAVMLKQEVDVVFQENEVMVLAVDHIRRL QLINGRTGQIDYLTEAQVSCCCLSPHLQYIAFGDENGAIEILELVNNRIF QSRFQHKKTVWHIQFTADEKTLISSSDDAEIQVWNWQLDKCIFLRGHQET VKDFRLLKNSRLLSWSFDGTVKVWNIITGNKEKDFVCHQGTVLSCDISHD ATKFSSTSADKTAKIWSFDLLLPLHELRGHNGCVRCSAFSVDSTLLATGD DNGEIRIWNVSNGELLHLCAPLSEEGAATHGGWVTDLCFSPDGKMLISAG GYIKWWNVVTGESSQTFYTNGTNLKKIHVSPDFKTYVTVDNLGILYILQT LE
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分子量
143 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
APAF1 (Apoptosis protein activating factor 1) is a pivotal component of the apoptosome, a multi-protein complex that plays a crucial role in the intrinsic pathway of apoptosis, or programmed cell death. This protein is vital for cellular homeostasis and response to various stressors, including DNA damage and oxidative stress. Mutations or alterations in APAF1 expression have been implicated in various diseases, particularly cancer, where dysregulation of apoptosis contributes to tumorigenesis. Research has increasingly focused on the recombinant expression of APAF1 to enable detailed studies of its structure, function, and interaction with other apoptotic factors. By producing recombinant APAF1, scientists can analyze its conformational changes, investigate its role in caspase activation, and explore potential therapeutic strategies aimed at reactivating apoptotic pathways in cancer cells. The ability to generate purified APAF1 in sufficient quantities also facilitates high-resolution structural studies using techniques such as X-ray crystallography and cryo-electron microscopy, enhancing our understanding of its molecular mechanisms. Overall, studies on recombinant APAF1 offer significant insights into apoptosis regulation and the potential development of novel cancer therapies targeting apoptotic dysregulation.
