Analytical Data
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Gene name
AIF
- Application
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Alternative Names
AIF；G1；IBA1；Allograft inflammatory factor 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O95831
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Expression Region
121-613aa
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AA Sequence
MGSSHHHHHHSSGLVPRGSHMEEVPQDKAPSHVPFLLIGGGTAAFAAARS IRARDPGARVLIVSEDPELPYMRPPLSKELWFSDDPNVTKTLRFKQWNGK ERSIYFQPPSFYVSAQDLPHIENGGVAVLTGKKVVQLDVRDNMVKLNDGS QITYEKCLIATGGTPRSLSAIDRAGAEVKSRTTLFRKIGDFRSLEKISRE VKSITIIGGGFLGSELACALGRKARALGTEVIQLFPEKGNMGKILPEYLS NWTMEKVRREGVKVMPNAIVQSVGVSSGKLLIKLKDGRKVETDHIVAAVG LEPNVELAKTGGLEIDSDFGGFRVNAELQARSNIWVAGDAACFYDIKLGR RRVEHHDHAVVSGRLAGENMTGAAKPYWHQSMFWSDLGPDVGYEAIGLVD SSLPTVGVFAKATAQDNPKSATEQSGTGIRSESETESEASEITIPPSTPA VPQAPVQGEDYGKGVIFYLRDKVVVGIVLWNIFNRMPIARKIIKDGEQHE DLNEVAKLFNIHED
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Molecular Weight
67 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
AIF (Apoptosis-Inducing Factor) is a mitochondrial protein that plays a pivotal role in mediating apoptosis and necrosis in response to cellular stress. Initially discovered for its involvement in programmed cell death, AIF has garnered significant attention due to its dual function in cell survival and death pathways. Research has shown that AIF can translocate from mitochondria to the nucleus during apoptosis, where it interacts with nuclear components to induce chromatin condensation and DNA fragmentation. Furthermore, AIF’s role extends beyond apoptosis, being implicated in various pathological conditions, including neurodegenerative diseases and cancer. The interest in AIF has propelled studies focused on its structural characteristics, post-translational modifications, and interactions with other proteins. Understanding the molecular mechanisms underlying AIF's actions not only sheds light on fundamental aspects of cellular regulation but also paves the way for potential therapeutic strategies aimed at modulating its activity in diseases characterized by dysregulated cell death. The recombination and manipulation of AIF have become a focal point in research, enabling scientists to explore its functions in detail and assess its potential as a biomarker or target for drug development. This growing body of work signifies the importance of AIF in cellular biology and its relevance in developing interventions for diseases associated with cell death dysregulation.
