Analytical Data
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Gene name
THRAP5
- Application
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Alternative Names
Med16; Thrap5; Mediator of RNA polymerase II transcription subunit 16; Mediator complex subunit 16; Thyroid hormone receptor-associated Protein 5; Thyroid hormone receptor-associated Protein complex 95 kDa component; Trap95; Vitamin D3 receptor-interacting Protein complex 92 kDa component; DRIP92
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q6PGF3
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Expression Region
1-860 aa
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AA Sequence
MCDLRRPAAGGMMDLAYVCEWEKWSKSTHCPSVPLACAWSCRNLIAFTMDLRSDDQDLTRMIHILDTEHPWDLHSIPSEHHEAITCLEWDQSGSRLLSADADGQIKCWSMADHLANSWESSVGSLVEGDPIVALSWLHNGVKLALHVEKSGASSFGEKFSRVKFSPSLTLFGGKPMEGWIAVTVSGLVTVSLLKPSGQVLTSTESLCRLRGRVALADIAFTGGGNIVVATADGSSASPVQFYKVCVSVVSEKCRIDTEILPSLFMRCTTDLNRKDKFPAITHLKFLARDMSEQVLLCASSQTSSIVECWSLRKEGLPVNNIFQQISPVVGDKQPTILKWRILSATNDLDRVSAVALPKLPISLTNTDLKVASDTQFYPGLGLALAFHDGSVHIVHRLSLQTMAVFYSSAAPRPVDEPAMKRPRTAGPAVHLKAMQLSWTSLALVGIDSHGKLSVLRLSPSMGHPLEVGLALRHLLFLLEYCMVTGYDWWDILLHVQPSMVQSLVEKLHEEYTRQTAALQQVLSTRILAMKASLCKLSPCTVTRVCDYHTKLFLIAISSTLKSLLRPHFLNTPDKSPGDRLTEICTKITDVDIDKVMINLKTEEFVLDMNTLQALQQLLQWVGDFVLYLLASLPNQGSLLRPGHSFLRDGTSLGMLRELMVVIRIWGLLKPSCLPVYTATSDTQDSMSLLFRLLTKLWICFPSTGPCSVWVLLGWQPLPGRDEGPASEPDEALVDECCLLPSQLLIPSLDWLPASDGLVSRLQPKQPLRLQFGRAPTLPGSAATLQLDGLARAPGQPKIDHLRRLHLGACPTEECKACTRCGCVTMLKSPNRTTAVKQWEQRWIKNCLCGGLWWRVPLSYP
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Molecular Weight
121.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
THRAP5 (Thyroid Hormone Receptor Associated Protein 5) is a key protein implicated in the regulation of gene expression and various cellular processes. It is part of the transcriptional co-regulators that interact with nuclear hormone receptors, influencing their ability to modulate target gene activity in response to thyroid hormones. Due to its role in cellular mechanisms such as proliferation, differentiation, and apoptosis, THRAP5 has garnered attention in various fields, including cancer research and metabolic disorders. Studies have shown that alterations in THRAP5 expression can affect tumor progression and response to treatments in different cancers, suggesting its potential as a biomarker or therapeutic target. Therefore, the generation of recombinant THRAP5 proteins is essential for further elucidating its biological functions, interaction partners, and structural characteristics. Utilizing recombinant techniques allows for the production of purified THRAP5 protein, facilitating in vitro studies such as binding assays, functional assays, and structural analyses. This research not only enhances our understanding of THRAP5's role in normal physiological functions but also its implications in pathological conditions, thereby paving the way for novel therapeutic approaches that may leverage THRAP5 modulation in disease contexts.
