Analytical Data
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基因名
TNKS1
- Application
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别名
TNKS1；PARP5A；PARPL；TIN1；Poly [ADP-ribose] polymerase tankyrase-1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O95271
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表达区间
1001-1327aa
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氨基酸序列
ELAVGGASNAGDGAAGTERKEGEVAGLDMNISQFLKSLGLEHLRDIFETE QITLDVLADMGHEELKEIGINAYGHRHKLIKGVERLLGGQQGTNPYLTFH CVNQGTILLDLAPEDKEYQSVEEEMQSTIREHRDGGNAGGIFNRYNVIRI QKVVNKKLRERFCHRQKEVSEENHNHHNERMLFHGSPFINAIIHKGFDER HAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYICHRQML FCRVTLGKSFLQFSTMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQA YPEYLITYQIMKPEAPSQTATAAEQKT
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分子量
63 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TNKS1 (Tankyrase 1) is a member of the poly(ADP-ribose) polymerase (PARP) family, primarily known for its role in regulating cellular processes such as telomere maintenance, Wnt signaling, and DNA repair through ADP-ribosylation. Research on TNKS1 has gained momentum due to its implications in cancer biology, particularly as it is often overexpressed in various malignancies, contributing to tumor progression and resistance to therapies. The enzyme catalyzes the addition of poly(ADP-ribose) chains to target proteins, influencing their stability and interactions, which plays a significant role in cellular signaling pathways. The development of TNKS1 inhibitors has emerged as a promising therapeutic strategy, targeting its enzymatic activity to disrupt pathological cellular processes. Reconstructing functional TNKS1 protein is essential for elucidating its biochemical properties and understanding its substrate interactions. Recent studies have focused on producing and characterizing recombinant TNKS1 proteins to analyze their structure and function, providing insights into the mechanisms of action and identifying novel substrates and regulators. These findings not only enhance our understanding of TNKS1's role in cellular functions but also pave the way for potential therapeutic interventions, underscoring the importance of TNKS1 research in the context of cancer treatment and cell biology.
