Analytical Data
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Gene name
EIF3S7
- Application
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Alternative Names
eIF 3 zeta; eIF-3-zeta; eIF3 p66; eIF3 zeta; eIF3d; EIF3D_HUMAN; eIF3p66; EIF3S7; Eukaryotic translation initiation factor 3 subunit 7; Eukaryotic translation initiation factor 3 subunit 7 zeta 66/67kDa; Eukaryotic translation initiation factor 3 subunit D; MGC126526
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O15371
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Expression Region
1-548aa
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AA Sequence
MAKFMTPVIQDNPSGWGPCAVPEQFRDMPYQPFSKGDRLGKVADWTGATYQDKRYTNKYSSQFGGGSQYAYFHEEDESSFQLVDTARTQKTAYQRNRMRFAQRNLRRDKDRRNMLQFNLQILPKSAKQKERERIRLQKKFQKQFGVRQKWDQKSQKPRDSSVEVRSDWEVKEEMDFPQLMKMRYLEVSEPQDIECCGALEYYDKAFDRITTRSEKPLRSIKRIFHTVTTTDDPVIRKLAKTQGNVFATDAILATLMSCTRSVYSWDIVVQRVGSKLFFDKRDNSDFDLLTVSETANEPPQDEGNSFNSPRNLAMEATYINHNFSQQCLRMGKERYNFPNPNPFVEDDMDKNEIASVAYRYRRWKLGDDIDLIVRCEHDGVMTGANGEVSFINIKTLNEWDSRHCNGVDWRQKLDSQRGAVIATELKNNSYKLARWTCCALLAGSEYLKLGYVSRYHVKDSSRHVILGTQQFKPNEFASQINLSVENAWGILRCVIDICMKLEEGKYLILKDPNKQVIRVYSLPDGTFSSDEDEEEEEEEEEEEEEEET
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Molecular Weight
85.91 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EIF3S7 (Eukaryotic Translation Initiation Factor 3 Subunit 7) is a critical component of the eukaryotic translation initiation factor 3 (eIF3) complex, which plays a pivotal role in the regulation of protein synthesis. This protein is involved in the initiation phase of translation by facilitating the binding of mRNA to the ribosomal machinery. Research on EIF3S7 has gained significant attention, as its expression and function are implicated in various cellular processes, including cell proliferation, differentiation, and apoptosis. Dysregulation of EIF3S7 has been associated with several diseases, especially cancer, where altered protein synthesis can contribute to tumorigenesis and metastasis. The study of recombinant EIF3S7 protein allows for the exploration of its structural and functional properties, as well as its interactions with other components of the eIF3 complex and mRNA. Understanding the intricate mechanisms underlying EIF3S7's role in translation initiation can provide insights into potential therapeutic strategies that target protein synthesis in cancer therapy and other diseases linked to translation dysregulation. Additionally, recombinant EIF3S7 can serve as a valuable tool for high-throughput screening assays aimed at identifying small-molecule inhibitors that selectively target aberrant translation initiation pathways. Through the investigation of EIF3S7, researchers hope to elucidate the fundamental processes of translation initiation and their implications in health and disease.
