Analytical Data
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Gene name
EIF2B3
- Application
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Alternative Names
EI2BG_HUMAN; EIF 2B; eIF 2B GDP GTP exchange factor subunit gamma ; eIF-2B GDP-GTP exchange factor subunit gamma; eIF2B-gamma; Eif2b3; EIF2Bgamma ; Eukaryotic translation initiation factor 2B subunit 3 gamma; Eukaryotic translation initiation factor 2B; gamma; eukaryotic translation initiation factor 2B
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NR50
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Expression Region
1-452aa
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AA Sequence
MEFQAVVMAVGGGSRMTDLTSSIPKPLLPVGNKPLIWYPLNLLERVGFEEVIVVTTRDVQKALCAEFKMKMKPDIVCIPDDADMGTADSLRYIYPKLKTDVLVLSCDLITDVALHEVVDLFRAYDASLAMLMRKGQDSIEPVPGQKGKKKAVEQRDFIGVDSTGKRLLFMANEADLDEELVIKGSILQKHPRIRFHTGLVDAHLYCLKKYIVDFLMENGSITSIRSELIPYLVRKQFSSASSQQGQEEKEEDLKKKELKSLDIYSFIKEANTLNLAPYDACWNACRGDRWEDLSRSQVRCYVHIMKEGLCSRVSTLGLYMEANRQVPKLLSALCPEEPPVHSSAQIVSKHLVGVDSLIGPETQIGEKSSIKRSVIGSSCLIKDRVTITNCLLMNSVTVEEGSNIQGSVICNNAVIEKGADIKDCLIGSGQRIEAKAKRVNEVIVGNDQLMEI
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Molecular Weight
76.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
EIF2B3 is a crucial component of the eukaryotic initiation factor 2B (eIF2B) complex, which plays a pivotal role in the regulation of protein synthesis. This protein is essential for the exchange of GDP for GTP on eIF2, a key step in the initiation of mRNA translation. Abnormalities in EIF2B3 and other eIF2B subunits have been linked to several neurological disorders, particularly a group of conditions known as eIF2B-related leukodystrophies. These disorders are characterized by white matter degeneration in the brain, resulting in a range of symptoms, including cognitive decline and motor dysfunction. Research into EIF2B3 is critical to understanding the molecular mechanisms underlying these leukodystrophies, as mutations in the EIF2B3 gene can disrupt its normal function, leading to impaired protein synthesis. Furthermore, elucidating the structure and function of the EIF2B3 protein can provide insights into potential therapeutic strategies for these diseases. Recent advances in molecular biology and protein engineering techniques have facilitated the study of EIF2B3, allowing researchers to explore its interactions with other components of the translation machinery and its role in cellular stress response pathways. This research not only enhances our understanding of fundamental cellular processes but also holds promise for the development of targeted interventions in disorders linked to EIF2B3 dysfunction. As such, the study of EIF2B3 and its recombinant forms not only contributes to the field of molecular biology but also has significant implications for clinical research and therapeutic innovation in neurodegenerative diseases.
