Analytical Data
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基因名
TCP10
- Application
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别名
TCP10; TCP10A; T-complex Protein 10A homolog
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q12799
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表达区间
1-353 aa
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氨基酸序列
MLEGQLEAGE PKEGTHPEDP CPGAGAAMEK TPAAAEVPRE DSNAGEMPSL QQQITSLHQE LGRQQSLWAD IHRKLQSHMD ALRKQNRELR EELRGLQRQQ WEAGKKPAAS PHAGRESHTL ALEPAFGKIS HLSADEDTTP KYAGRKSQSA TLLGQRWSSN HLAPPKPMSL KTERINSGKT PPQEDREKSP PGRRQDRSPA PTGRPTPGAE RRGVSEDGKI MHPSSRSPQN SGGRKSPVQA SQATTLQEQT AAARGADRSS SVLGSSEGGF LSRVQADEFA SSAPDSAERQ NLPVNPPSSL EIAQAMDTKM KKEEVQEEKR HPKGKADDCR RSGFPSEFPG ALHAAPSRQD MGP
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分子量
64.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TCP10, or T-complex protein 10, is a member of the T-complex polypeptide family, which plays a crucial role in cellular processes such as protein folding, assembly, and transport. Its study has gained significant attention in the context of reproductive biology, particularly in spermatogenesis and fertilization, as TCP10 is expressed in the testes and is implicated in sperm motility and function. Research has shown that TCP10 acts as a chaperone, assisting in the proper folding of other proteins, and its dysregulation may lead to male infertility. Additionally, TCP10 has been associated with the T-complex locus on mouse chromosome 17, which is important for understanding genetic inheritance and variation. The exploration of TCP10's structure and function is critical for elucidating its biological roles and potential applications in therapeutic interventions for infertility and other reproductive disorders. Recent studies employ innovative techniques such as transgenic mouse models and protein interaction assays to dissect the molecular mechanisms involving TCP10. This research not only enhances our understanding of male reproductive health but also provides insights into the evolutionary significance of the T-complex and its proteins in mammals. Overall, TCP10 serves as a pivotal focus in reproductive research, offering promising avenues for future studies aimed at resolving fertility challenges and advancing reproductive technologies.
