Analytical Data
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Gene name
ZBTB45
- Application
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Alternative Names
ZBTB45; ZNF499; Zinc finger and BTB domain-containing Protein 45; Zinc finger Protein 499
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96K62
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Expression Region
1-511 aa
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AA Sequence
MAAAEAVHHIHLQNFSRSLLETLNGQRLGGHFCDVTVRIREASLRAHRCVLAAGSPFFQDKLLLGHSEIRVPPVVPAQTVRQLVEFLYSGSLVVAQGEALQVLTAASVLRIQTVIDECTQIIARARAPGTSAPTPLPTPVPPPLAPAQLRHRLRHLLAARPPGHPGAAHSRKQRQPARLQLPAPPTPAKAEGPDADPSLSAAPDDRGDEDDEESDDETDGEDGEGGGPGEGQAPPSFPDCAAGFLTAAADSACEEPPAPTGLADYSGAGRDFLRGAGSAEDVFPDSYVSTWHDEDGAVPEGCPTETPVQPDCILSGSRPPGVKTPGPPVALFPFHLGAPGPPAPPPSAPSGPAPAPPPAFYPTLQPEAAPSTQLGEVPAPSAAPTTAPSGTPARTPGAEPPTYECSHCRKTFSSRKNYTKHMFIHSGEKPHQCAVCWRSFSLRDYLLKHMVTHTGVRAFQCAVCAKRFTQKSSLNVHMRTHRPERAPCPACGKVFSHRALLERHLAAHPAP
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Molecular Weight
56.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ZBTB45, a member of the zinc finger and BTB domain-containing protein family, plays a significant role in various biological processes, including gene regulation and immune response. Initial studies have suggested its involvement in hematopoiesis and T-cell development, indicating its potential function in both normal physiological and pathological contexts, such as cancer and autoimmune diseases. The interest in ZBTB45 has grown due to its emerging role in regulating transcriptional activities through chromatin remodeling and interaction with various co-regulators. Research has indicated that ZBTB45 may act as a transcriptional silencer for certain genes, thereby influencing cellular differentiation and proliferation. Additionally, the characterization of recombinant ZBTB45 protein is crucial for elucidating its structure-function relationship and understanding its mechanisms of action at the molecular level. By producing this recombinant protein, researchers aim to investigate its binding affinity to DNA, interaction with other proteins, and downstream signaling pathways. The insights gained from these studies could have implications for developing therapeutic strategies targeting ZBTB45-related pathways in diseases, making it a focal point for ongoing research in molecular biology and medicine.
