Analytical Data
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Gene name
CH25H
- Application
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Alternative Names
CH25H；Cholesterol 25-hydroxylase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O95992
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Expression Region
1-272aa
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AA Sequence
MSCHNCSDPQVLCSSGQLFLQPLWDHLRSWEALLQSPFFPVIFSITTYVG FCLPFVVLDILCSWVPALRRYKIHPDFSPSAQQLLPCLGQTLYQHVMFVF PVTLLHWARSPALLPHEAPELLLLLHHILFCLLLFDMEFFVWHLLHHKVP WLYRTFHKVHHQNSSSFALATQYMSVWELFSLGFFDMMNVTLLGCHPLTT LTFHVVNIWLSVEDHSGYNFPWSTHRLVPFGWYGGVVHHDLHHSHFNCNF APYFTHWDKILGTLRTASVPAR
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CH25H, or cholesterol 25-hydroxylase, is an enzyme that plays a critical role in cholesterol metabolism and homeostasis. Its primary function is to convert cholesterol into 25-hydroxycholesterol, a bioactive lipid that influences various biological processes, including immune responses, inflammation, and lipid metabolism. Research has shown that CH25H is linked to various diseases, including atherosclerosis, metabolic syndrome, and certain autoimmune disorders. Advances in biotechnology have enabled the recombinant expression of CH25H, facilitating detailed studies of its structure-function relationships and biological activities. By producing CH25H as a recombinant protein, researchers can investigate its enzymatic mechanisms, regulatory pathways, and interactions with other molecules. These studies have the potential to reveal novel therapeutic targets and strategies for treating conditions associated with dysregulated cholesterol metabolism. Understanding the physiological and pathological roles of CH25H is crucial for uncovering its potential as a biomarker and therapeutic target in various diseases, thus driving the development of innovative interventions in cardiovascular and metabolic health.
