Analytical Data
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基因名
CH25H
- Application
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别名
CH25H；Cholesterol 25-hydroxylase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O95992
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表达区间
1-272aa
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氨基酸序列
MSCHNCSDPQVLCSSGQLFLQPLWDHLRSWEALLQSPFFPVIFSITTYVG FCLPFVVLDILCSWVPALRRYKIHPDFSPSAQQLLPCLGQTLYQHVMFVF PVTLLHWARSPALLPHEAPELLLLLHHILFCLLLFDMEFFVWHLLHHKVP WLYRTFHKVHHQNSSSFALATQYMSVWELFSLGFFDMMNVTLLGCHPLTT LTFHVVNIWLSVEDHSGYNFPWSTHRLVPFGWYGGVVHHDLHHSHFNCNF APYFTHWDKILGTLRTASVPAR
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CH25H, or cholesterol 25-hydroxylase, is an enzyme that plays a critical role in cholesterol metabolism and homeostasis. Its primary function is to convert cholesterol into 25-hydroxycholesterol, a bioactive lipid that influences various biological processes, including immune responses, inflammation, and lipid metabolism. Research has shown that CH25H is linked to various diseases, including atherosclerosis, metabolic syndrome, and certain autoimmune disorders. Advances in biotechnology have enabled the recombinant expression of CH25H, facilitating detailed studies of its structure-function relationships and biological activities. By producing CH25H as a recombinant protein, researchers can investigate its enzymatic mechanisms, regulatory pathways, and interactions with other molecules. These studies have the potential to reveal novel therapeutic targets and strategies for treating conditions associated with dysregulated cholesterol metabolism. Understanding the physiological and pathological roles of CH25H is crucial for uncovering its potential as a biomarker and therapeutic target in various diseases, thus driving the development of innovative interventions in cardiovascular and metabolic health.
