Analytical Data
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Gene name
EBF3
- Application
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Alternative Names
COE 3; COE3; COE3_HUMAN; Early B cell factor 3; Early B-cell factor 3; EBF 3; EBF-3; EBF3; O/E 2; O/E-2; O/E2; OE 2; OE-2; OE2; Olf 1/EBF like 2; Olf-1/EBF-like 2; Transcription factor COE3
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9H4W6
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Expression Region
1-596aa
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AA Sequence
MFGIQENIPR GGTTMKEEPL GSGMNPVRSW MHTAGVVDAN TAAQSGVGLA RAHFEKQPPS NLRKSNFFHF VLALYDRQGQ PVEIERTAFV DFVEKEKEPN NEKTNNGIHY KLQLLYSNGV RTEQDLYVRL IDSMTKQAIV YEGQDKNPEM CRVLLTHEIM CSRCCDKKSC GNRNETPSDP VIIDRFFLKF FLKCNQNCLK NAGNPRDMRR FQVVVSTTVN VDGHVLAVSD NMFVHNNSKH GRRARRLDPS EGTAPSYLEN ATPCIKAISP SEGWTTGGAT VIIIGDNFFD GLQVVFGTML VWSELITPHA IRVQTPPRHI PGVVEVTLSY KSKQFCKGAP GRFVYTALNE PTIDYGFQRL QKVIPRHPGD PERLPKEVLL KRAADLVEAL YGMPHNNQEI ILKRAADIAE ALYSVPRNHN QIPTLGNNPA HTGMMGVNSF SSQLAVNVSE TSQANDQVGY SRNTSSVSPR GYVPSSTPQQ SNYNTVSTSM NGYGSGAMAS LGVPGSPGFL NGSSANSPYG IVPSSPTMAA SSVTLPSNCS STHGIFSFSP ANVISAVKQK SAFAPVVRPQ ASPPPSCTSA NGNGLQAMSG LVVPPM
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Molecular Weight
64.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EBF3 (Early B-cell Factor 3) is a member of the EBF family of transcription factors, which play critical roles in the development and function of various cell types, particularly in the immune system. Research into EBF3 has gained momentum due to its involvement in B-cell differentiation and its potential implications in immune-related disorders and malignancies. Studies have identified EBF3 as a key regulator of genes involved in B-cell lineage commitment, influencing processes such as proliferation, survival, and response to signaling pathways. Notably, aberrations in EBF3 expression have been linked to several hematological conditions, including lymphomas and leukemias, suggesting its significance in oncogenesis. Moreover, the characterization of EBF3 as a repressor of certain transcriptional networks positions it as a vital component in maintaining cellular fidelity. The production of recombinant EBF3 protein facilitates detailed biochemical and structural studies, enabling researchers to investigate its functional mechanisms and interactions with other proteins, thereby enhancing the understanding of its role in B-cell biology and associated diseases. The exploration of EBF3 and its pathways could pave the way for novel therapeutic strategies targeting immune dysregulation and cancer, making it a promising candidate for future research and clinical applications.
