Cat: PAX2000-11889

Recombinant Human TCFL5 Protein,His

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Analytical Data

  • Gene name

    TCFL5

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    bHLHe82; CHA; Cha transcription factor; E2BP-1; E2BP1; Figlb; HPV-16 E2-binding Protein 1; MGC46135; TCFL 5; TCFL5; TCFL5_HUMAN; transcription factor-like 5 (basic helix-loop-helix); Transcription factor-like 5 Protein

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q9UL49

  • Expression Region

    1-500 aa

  • AA Sequence

    MSGPGPREPP PEAGAAGGEA AVEGAGGGDA ALGEPGLSFT TTDLSLVEMT EVEYTQLQHI LCSHMEAAAD GELETRLNSA LLAAAGPGAG AGGFAAGGQG GAAPVYPVLC PSALAADAPC LGHIDFQELR MMLLSEAGAA EKTSGGGDGA RARADGAAKE GAGAAAAAAG PDGAPEARAK PAVRVRLEDR FNSIPAEPPP APRGPEPPEP GGALNNLVTL IRHPSELMNV PLQQQNKCTA LVKNKTAATT TALQFTYPLF TTNACSTSGN SNLSQTQSSS NSCSVLEAAK HQDIGLPRAF SFCYQQEIES TKQTLGSRNK VLPEQVWIKV GEAALCKQAL KRNRSRMRQL DTNVERRALG EIQNVGEGAT ATQGAWQSSE SSQANLGEQA QSGPQGGRSQ RRERHNRMER DRRRRIRICC DELNLLVPFC NAETDKATTL QWTTAFLKYI QERHGDSLKK EFESVFCGKT GRRLKLTRPD SLVTCPAQGS LQSSPSMEIK

  • Molecular Weight

    52.6 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

TCFL5, or T Cell Factor-Like 5, is a crucial protein that plays a pivotal role in various biological processes, including immune response regulation and cellular differentiation. The study of TCFL5 is of particular interest due to its involvement in the Wnt signaling pathway, which is essential for embryonic development and stem cell maintenance. Dysregulation of this pathway can lead to several diseases, including cancer, making TCFL5 a potential target for therapeutic interventions. Researchers have been investigating the structural and functional characteristics of recombinant TCFL5 proteins to better understand their role in these pathways. By employing techniques such as recombinant DNA technology, scientists can produce large quantities of TCFL5, allowing for detailed biochemical and biophysical characterization. This includes investigating its interactions with other proteins and understanding how mutations may affect its function. The insights gained from TCFL5 research not only enhance our understanding of immune and developmental biology but also hold promise for developing novel strategies in disease treatment and management. Furthermore, TCFL5's potential as a biomarker in cancer diagnosis is an active area of research, highlighting the translational significance of studying this protein. As a result, TCFL5 represents a compelling focus for ongoing studies aimed at deciphering its complex roles in health and disease.

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