Analytical Data
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Gene name
TCF3
- Application
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Alternative Names
Amida; INO80 complex subunit F; INO80F; Protein FB1; TCF3 (E2A) fusion partner (in childhood Leukemia); TCF3 fusion partner; TFPT; TFPT_HUMAN
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P0C1Z6
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Expression Region
1-253 aa
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AA Sequence
MELEQREGTM AAVGFEEFSA PPGSELALPP LFGGHILESE LETEVEFVSG GLGGSGLRER DEEEEAARGR RRRQRELNRR KYQALGRRCR EIEQVNERVL NRLHQVQRIT RRLQQERRFL MRVLDSYGDD YRASQFTIVL EDEGSQGTDA PTPGNAENEP PEKETLSPPR RTPAPPEPGS PAPGEGPSGR KRRRVPRDGR RAGNALTPEL APVQIKVEED FGFEADEALD SSWVSRGPDK LLPYPTLASP ASD
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Molecular Weight
28.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
TCF3, also known as E2A, is a transcription factor that plays a critical role in hematopoiesis and lymphocyte development. Its importance in immunology and cancer biology has led to extensive research into its function and regulatory mechanisms. TCF3 is involved in various physiological processes, including cell proliferation, differentiation, and survival, primarily by regulating gene expression in response to developmental cues. In particular, TCF3 is known to form complexes with other proteins, influencing the transcriptional activity essential for B-cell and T-cell lineages. However, mutations and aberrant expression of TCF3 have been implicated in several hematological malignancies, notably precursor B-cell acute lymphoblastic leukemia (ALL), where TCF3 is often found to be involved in translocations that disrupt normal function. Studying recombinant TCF3 protein allows researchers to investigate its structural and functional properties, providing insights into its role in gene regulation and potential therapeutic targets. This understanding may pave the way for innovative strategies in treating TCF3-related disorders and enhancing our comprehension of oncogenic mechanisms linked to transcription factors. The ability to manipulate TCF3 at the molecular level is, therefore, a promising area of inquiry for both basic and applied biomedical research.
