Analytical Data
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Gene name
EPN1
- Application
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Alternative Names
EPN1；Epsin-1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9Y6I3
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Expression Region
1-576aa
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AA Sequence
MSTSSLRRQM KNIVHNYSEA EIKVREATSN DPWGPSSSLM SEIADLTYNV VAFSEIMSMI WKRLNDHGKN WRHVYKAMTL MEYLIKTGSE RVSQQCKENM YAVQTLKDFQ YVDRDGKDQG VNVREKAKQL VALLRDEDRL REERAHALKT KEKLAQTATA SSAAVGSGPP PEAEQAWPQS SGEEELQLQL ALAMSKEEAD QPPSCGPEDD AQLQLALSLS REEHDKEERI RRGDDLRLQM AIEESKRETG GKEESSLMDL ADVFTAPAPA PTTDPWGGPA PMAAAVPTAA PTSDPWGGPP VPPAADPWGG PAPTPASGDP WRPAAPAGPS VDPWGGTPAP AAGEGPTPDP WGSSDGGVPV SGPSASDPWT PAPAFSDPWG GSPAKPSTNG TTAAGGFDTE PDEFSDFDRL RTALPTSGSS AGELELLAGE VPARSPGAFD MSGVRGSLAE AVGSPPPAAT PTPTPPTRKT PESFLGPNAA LVDLDSLVSR PGPTPPGAKA SNPFLPGGGP ATGPSVTNPF QPAPPATLTL NQLRLSPVPP VPGAPPTYIS PLGGGPGLPP MMPPGPPAPN TNPFLL
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
EPN1, or Epsin 1, is an important protein involved in receptor endocytosis and the regulation of membrane trafficking. It plays a crucial role in clathrin-mediated endocytosis, where it facilitates the internalization of various cell surface receptors, including those involved in signaling pathways. The study of EPN1 has gained significance due to its potential implications in various diseases, including cancer and neurodegenerative disorders. Mutations or dysregulation of EPN1 can lead to altered cellular uptake mechanisms, thus affecting signal transduction and cellular communication. Research focusing on the recombinant expression of EPN1 allows scientists to analyze its structure-function relationships and interactions with other cellular proteins. By producing EPN1 in a controlled laboratory environment, researchers can explore its binding affinities, post-translational modifications, and its role in the cellular internalization processes at a molecular level. This understanding not only provides insights into the fundamental biology of endocytosis but also opens up avenues for therapeutic interventions targeting EPN1 and related pathways in disease contexts. As such, EPN1 recombinant protein research stands at the intersection of cell biology, biochemistry, and translational medicine, highlighting its relevance in both basic and applied scientific fields.
