Analytical Data
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Gene name
FOXO3
- Application
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Alternative Names
FOXO3；FKHRL1；FOXO3A；Forkhead box Protein O3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O43524
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Expression Region
1-673aa
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AA Sequence
MAEAPASPAPLSPLEVELDPEFEPQSRPRSCTWPLQRPELQASPAKPSGE TAADSMIPEEEDDEDDEDGGGRAGSAMAIGGGGGSGTLGSGLLLEDSARV LAPGGQDPGSGPATAAGGLSGGTQALLQPQQPLPPPQPGAAGGSGQPRKC SSRRNAWGNLSYADLITRAIESSPDKRLTLSQIYEWMVRCVPYFKDKGDS NSSAGWKNSIRHNLSLHSRFMRVQNEGTGKSSWWIINPDGGKSGKAPRRR AVSMDNSNKYTKSRGRAAKKKAALQTAPESADDSPSQLSKWPGSPTSRSS DELDAWTDFRSRTNSNASTVSGRLSPIMASTELDEVQDDDAPLSPMLYSS SASLSPSVSKPCTVELPRLTDMAGTMNLNDGLTENLMDDLLDNITLPPSQ PSPTGGLMQRSSSFPYTTKGSGLGSPTSSFNSTVFGPSSLNSLRQSPMQT IQENKPATFSSMSHYGNQTLQDLLTSDSLSHSDVMMTQSDPLMSQASTAV SAQNSRRNVMLRNDPMMSFAAQPNQGSLVNQNLLHHQHQTQGALGGSRAL SNSVSNMGLSESSSLGSAKHQQQSPVSQSMQTLSDSLSGSSLYSTSANLP VMGHEKFPSDLDLDMFNGSLECDMESIIRSELMDADGLDFNFDSLISTQN VVGLNVGNFTGAKQASSQSWVPG
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Molecular Weight
100 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
FOXO3, a member of the Forkhead box O (FoxO) transcription factor family, plays a crucial role in various cellular processes including metabolism, stress resistance, and apoptosis. Its significance has garnered substantial interest, particularly in the context of aging and age-related diseases. Research indicates that FOXO3 is associated with longevity, as certain genetic variants of the FOXO3 gene have been linked to an increased lifespan in humans. The ability of FOXO3 to regulate key pathways involved in cell survival and proliferation makes it a potential therapeutic target for age-related disorders and cancer. Recombination and expression of FOXO3 protein facilitate the investigation of its functional roles and regulatory mechanisms in cellular responses to stress and metabolic challenges. Current studies focus on understanding how FOXO3 mediates insulin sensitivity, oxidative stress responses, and autophagy, all of which are vital for maintaining cellular homeostasis. By examining the reconstituted FOXO3 protein, researchers aim to delineate its interaction with other signaling pathways and proteins, providing insights into its developmental, metabolic, and oncogenic implications. This research not only enhances our comprehension of FOXO3's biological significance but also opens avenues for developing innovative strategies in regenerative medicine and targeted therapies against aging and cancer. As such, the exploration of FOXO3 recombinant protein stands at the forefront of scientific endeavors to unlock the mechanisms of durability and resilience in cellular systems.
