Analytical Data
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基因名
LIF
- Application
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别名
LIF；HILDA；Leukemia inhibitory factor
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P15018
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表达区间
23-202aa
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氨基酸序列
SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF
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分子量
20 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
LIF (Leukemia Inhibitory Factor) is a multifunctional cytokine belonging to the IL-6 family, renowned for its roles in regulating diverse biological processes such as cell differentiation, immunity, and embryonic development. Its unique ability to maintain pluripotency in embryonic stem cells (ESCs) has made LIF a subject of intense investigation in developmental biology and regenerative medicine. The therapeutic potential of LIF is underscored by its involvement in cancer biology, where it can exhibit both tumor-promoting and suppressive effects depending on the context. The recombinant production of LIF has become increasingly relevant as it allows for the exploration of its biological functions and therapeutic applications in a controlled manner. Advances in molecular cloning techniques and protein expression systems have facilitated the generation of high yields of biologically active LIF, opening avenues for its use in stem cell research and potential clinical applications, such as regenerative therapies and cancer treatment. Recent studies have focused on understanding the signaling pathways activated by LIF and its interaction with other cytokines, providing insights into its complex roles in cellular dynamics. As research progresses, recombinant LIF has emerged as a pivotal tool in elucidating the mechanisms governing stem cell behavior and developing novel therapeutic strategies aimed at tissue repair and regeneration.
