Analytical Data
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Gene name
DNAJC5B
- Application
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Alternative Names
DNAJC5BDnaJ homolog subfamily C member 5B; Cysteine string protein beta; CSP-beta
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9UF47
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Expression Region
1-199
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AA Sequence
MACNIPNQRQ RTLSTTGEAL YEILGLHKGA SNEEIKKTYR KLALKHHPDK NPDDPAATEK FKEINNAHAI LTDISKRSIY DKYGSLGLYV AEQFGDENVN TYFMLSSWWA KALFVIVGLL TGCYFCCCLC CCCNCCCGHC RPESSVPEED FYVSPEDLEE QIKSDMEKDV DFPVFLQPTN ANEKTQLIKE GSRSYCTDS
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Molecular Weight
22.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DNAJC5B is a member of the DnaJ family of proteins, which are known for their roles as molecular chaperones involved in protein folding and stress responses. The research on DNAJC5B has gained momentum due to its implications in neurodegenerative diseases and its essential role in cellular processes such as autophagy and protein degradation. Mutations in the DNAJC5B gene have been linked to conditions like adult-onset neuronal ceroid lipofuscinosis (CLN4), a disorder characterized by progressive neurodegeneration and cognitive decline. Understanding the functional properties of DNAJC5B and its interactions with other cellular components is crucial for elucidating the mechanisms underlying these diseases. Recent studies have focused on the structural characteristics and the chaperone activity of DNAJC5B, providing insights into its functional dynamics. Additionally, the development of recombinant DNAJC5B proteins has enabled researchers to conduct in vitro studies, facilitating a better understanding of its role in protein misfolding and aggregation. Through these investigations, the potential therapeutic applications of DNAJC5B targeting in neurodegenerative diseases are being explored, highlighting its significance in the field of molecular biology and medicine.
