Analytical Data
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Gene name
DHRS1
- Application
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Alternative Names
1110029G07Rik; AW112170; D14Ertd484e; dehydrogenase/reductase (SDR family) member 1; Dehydrogenase/reductase SDR family member 1; DHRS; Dhrs1; DHRS1_HUMAN; FLJ25430
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96LJ7
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Expression Region
2-313aa
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AA Sequence
AAPMNGQVC VVTGASRGIG RGIALQLCKA GATVYITGRH LDTLRVVAQE AQSLGGQCVP VVCDSSQESE VRSLFEQVDR EQQGRLDVLV NNAYAGVQTI LNTRNKAFWE TPASMWDDIN NVGLRGHYFC SVYGARLMVP AGQGLIVVIS SPGSLQYMFN VPYGVGKAAC DKLAADCAHE LRRHGVSCVS LWPGIVQTEL LKEHMAKEEV LQDPVLKQFK SAFSSAETTE LSGKCVVALA TDPNILSLSG KVLPSCDLAR RYGLRDVDGR PVQDYLSLSS VLSHVSGLGW LASYLPSFLR VPKWIIALYT SKF
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Molecular Weight
33.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DHRS1 (dehydrogenase/reductase member 1) is an enzyme that plays a crucial role in the metabolism of various substrates, including steroids and retinoids. Recent research has highlighted its significance in cellular processes such as apoptosis, signal transduction, and the regulation of lipid and carbohydrate metabolism. Notably, DHRS1 is involved in the reduction of 9-cis-retinal to retinol, a key step in the visual cycle. The enzyme's expression levels have been linked to several diseases, including metabolic disorders and certain cancers, making it a potential biomarker for these conditions. Additionally, studies have indicated that DHRS1 may have protective roles in oxidative stress, further underscoring its importance in maintaining cellular homeostasis. Given its multifaceted role in metabolic pathways and disease mechanisms, the recombinant expression and characterization of DHRS1 protein have gained attention. This research aims to provide insights into the enzyme's structure-function relationships and its potential therapeutic applications. Understanding the biological functions and regulatory mechanisms of DHRS1 could pave the way for the development of targeted treatments for diseases associated with its dysfunction. Consequently, the recombinant production of DHRS1 not only facilitates detailed biochemical studies but also lays the groundwork for future drug discovery efforts targeting the pathways in which this enzyme is involved.
