Analytical Data
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基因名
SPAST
- Application
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别名
Spastic paraplegia 4 protein
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9UBP0
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表达区间
317-616 aa
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氨基酸序列
FRNVDSNLANLIMNEIVDNGTAVKFDDIAGQDLAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFGDTTV
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分子量
40.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SPAST (Spastin) is a crucial protein encoded by the SPAST gene, primarily associated with hereditary spastic paraplegia (HSP), a neurodegenerative disorder characterized by progressive weakness and stiffness of the lower limbs. The importance of SPAST lies in its role in the severing of microtubules, which are essential for proper neuronal function and axonal transport. Mutations in the SPAST gene disrupt these cellular processes, leading to the degeneration of motor neurons. Research on SPAST recombinant protein has gained momentum as it can serve as a valuable tool to elucidate the molecular mechanisms underlying HSP and aid in the development of therapeutic strategies. By producing and characterizing recombinant SPAST, researchers aim to better understand its functional domains, interactions with other cellular components, and the impact of specific mutations on its activity. Furthermore, studying the effects of SPAST dysfunction at the cellular level can provide insights into the broader implications of microtubule dynamics in neurodegenerative diseases. Thus, SPAST recombinant protein research stands at the intersection of molecular biology and clinical neuroscience, promising advancements in the understanding and potential treatment of HSP and similar conditions.
