Analytical Data
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Gene name
POLR3C
- Application
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Alternative Names
POLR3C；DNA-directed RNA polymerase III subunit RPC7
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BUI4
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Expression Region
1-534aa
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AA Sequence
MTQAEIKLCSLLLQEHFGEIVEKIGVHLIRTGSQPLRVIAHDTGTSLDQVKKALCVLVQHNLVSYQVHKRGVVEYEAQCSRVLRMLRYPRYIYTTKTLYSDTGELIVEELLLNGKLTMSAVVKKVADRLTETMEDGKTMDYAEVSNTFVRLADTHFVQRCPSVPTTENSDPGPPPPAPTLVINEKDMYLVPKLSLIGKGKRRRSSDEDAAGEPKAKRPKYTTDNKEPIPDDGIYWQANLDRFHQHFRDQAIVSAVANRMDQTSSEIVRTMLRMSEITTSSSAPFTQPLSSNEIFRSLPVGYNISKQVLDQYLTLLADDPLEFVGKSGDSGGGMYVINLHKALASLATATLESVVQERFGSRCARIFRLVLQKKHIEQKQVEDFAMIPAKEAKDMLYKMLSENFMSLQEIPKTPDHAPSRTFYLYTVNILSAARMLLHRCYKSIANLIERRQFETKENKRLLEKSQRVEAIIASMQATGAEEAQLQEIEEMITAPERQQLETLKRNVNKLDASEIQVDETIFLLESYIECTMKRQ
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Molecular Weight
76.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
POLR3C, a key component of the RNA polymerase III (Pol III) complex, plays a crucial role in synthesizing various small RNAs, including tRNAs, 5S rRNA, and other non-coding RNAs essential for cellular functions. Research into POLR3C has gained significance due to its involvement in both fundamental cellular processes and pathological conditions, including cancer and autoimmune diseases. Mutations or dysregulation of POLR3C can disrupt normal RNA synthesis, leading to impaired cellular activities and contributing to disease progression. Moreover, POLR3C has emerged as a target for understanding the molecular mechanisms of certain neurological disorders, where altered RNA metabolism is a critical factor. The study of recombinant POLR3C protein facilitates insights into its structural and functional attributes, enabling the exploration of its interactions with various transcription factors and other components of the transcription machinery. By employing techniques such as X-ray crystallography, cryo-electron microscopy, and biophysical analyses, researchers aim to elucidate the intricate regulatory mechanisms underlying Pol III transcription. Ultimately, gaining a deeper understanding of POLR3C's role will enhance our knowledge of gene expression regulation and its implications in health and disease, providing potential avenues for therapeutic interventions.
