Analytical Data
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基因名
POLR3C
- Application
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别名
POLR3C；DNA-directed RNA polymerase III subunit RPC7
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9BUI4
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表达区间
1-534aa
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氨基酸序列
MTQAEIKLCSLLLQEHFGEIVEKIGVHLIRTGSQPLRVIAHDTGTSLDQVKKALCVLVQHNLVSYQVHKRGVVEYEAQCSRVLRMLRYPRYIYTTKTLYSDTGELIVEELLLNGKLTMSAVVKKVADRLTETMEDGKTMDYAEVSNTFVRLADTHFVQRCPSVPTTENSDPGPPPPAPTLVINEKDMYLVPKLSLIGKGKRRRSSDEDAAGEPKAKRPKYTTDNKEPIPDDGIYWQANLDRFHQHFRDQAIVSAVANRMDQTSSEIVRTMLRMSEITTSSSAPFTQPLSSNEIFRSLPVGYNISKQVLDQYLTLLADDPLEFVGKSGDSGGGMYVINLHKALASLATATLESVVQERFGSRCARIFRLVLQKKHIEQKQVEDFAMIPAKEAKDMLYKMLSENFMSLQEIPKTPDHAPSRTFYLYTVNILSAARMLLHRCYKSIANLIERRQFETKENKRLLEKSQRVEAIIASMQATGAEEAQLQEIEEMITAPERQQLETLKRNVNKLDASEIQVDETIFLLESYIECTMKRQ
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分子量
76.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
POLR3C, a key component of the RNA polymerase III (Pol III) complex, plays a crucial role in synthesizing various small RNAs, including tRNAs, 5S rRNA, and other non-coding RNAs essential for cellular functions. Research into POLR3C has gained significance due to its involvement in both fundamental cellular processes and pathological conditions, including cancer and autoimmune diseases. Mutations or dysregulation of POLR3C can disrupt normal RNA synthesis, leading to impaired cellular activities and contributing to disease progression. Moreover, POLR3C has emerged as a target for understanding the molecular mechanisms of certain neurological disorders, where altered RNA metabolism is a critical factor. The study of recombinant POLR3C protein facilitates insights into its structural and functional attributes, enabling the exploration of its interactions with various transcription factors and other components of the transcription machinery. By employing techniques such as X-ray crystallography, cryo-electron microscopy, and biophysical analyses, researchers aim to elucidate the intricate regulatory mechanisms underlying Pol III transcription. Ultimately, gaining a deeper understanding of POLR3C's role will enhance our knowledge of gene expression regulation and its implications in health and disease, providing potential avenues for therapeutic interventions.
