Analytical Data
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基因名
DDX59
- Application
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别名
DDX 59; DDX59; DDX59_HUMAN; DEAD (Asp-Glu-Ala-Asp) box polypeptide 59 ; DEAD box protein 59; DKFZP564B1023; OTTHUMP00000033840; OTTHUMP00000033841; Probable ATP-dependent RNA helicase DDX59; RP11-92G12.2
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q5T1V6
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表达区间
1-619aa
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氨基酸序列
MFVPRSLKIKRNANDDGKSCVAKIIKPDPEDLQLDKSRDVPVDAVATEAATIDRHISESCPFPSPGGQLAEVHSVSPEQGAKDSHPSEEPVKSFSKTQRWAEPGEPICVVCGRYGEYICDKTDEDVCSLECKAKHLLQVKEKEEKSKLSNPQKADSEPESPLNASYVYKEHPFILNLQEDQIENLKQQLGILVQGQEVTRPIIDFEHCSLPEVLNHNLKKSGYEVPTPIQMQMIPVGLLGRDILASADTGSGKTAAFLLPVIMRALFESKTPSALILTPTRELAIQIERQAKELMSGLPRMKTVLLVGGLPLPPQLYRLQQHVKVIIATPGRLLDIIKQSSVELCGVKIVVVDEADTMLKMGFQQQVLDILENIPNDCQTILVSATIPTSIEQLASQLLHNPVRIITGEKNLPCANVRQIILWVEDPAKKKKLFEILNDKKLFKPPVLVFVDCKLGADLLSEAVQKITGLKRISIHSEKSQIERKNILKGLLEGDYEVVVSTGVLGRGLDLISVRLVVNFDMPSSMDEYVHQIGRVGRLGQNGTAITFINNNSKRLFWDIAKRVKPTGSILPPQLLNSPYLHDQKRKEQQKDKQTQNDLVTGANLMDIIRKHDKSNSQK
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分子量
95.3 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DDX59, a member of the DEAD-box helicase family, has garnered attention in recent years due to its involvement in various cellular processes, including RNA metabolism, translation regulation, and stress response mechanisms. This protein is characterized by its ATP-dependent RNA unwinding activity, which plays a critical role in modulating gene expression and RNA splicing. Dysregulation of DDX59 has been implicated in several diseases, including cancer and neurological disorders, making it a potential biomarker and therapeutic target. Research on recombinant DDX59 protein has focused on understanding its structural and functional properties, as well as elucidating its interaction with RNA and other cellular partners. By expressing DDX59 in a recombinant system, researchers aim to obtain sufficient quantities of the protein for biochemical assays, crystallography, and functional studies. These investigations are essential for uncovering the mechanistic roles of DDX59 in cellular functions and its potential implications in disease pathology. Furthermore, studying DDX59's interactions with small molecules or RNA can pave the way for the development of novel therapeutic strategies aimed at modulating its activity in pathological contexts.
