Cat: PA1000-7834

Recombinant Human AMIGO1 Protein,His

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Analytical Data

  • Gene name

    AMIGO1

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    AMIGO1;ALI2;AMIGO;KIAA1163;Amphoterin-induced Protein 1

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q86WK6

  • Expression Region

    1-493aa

  • AA Sequence

    MHPHRDPRGLWLLLPSLSLLLFEVARAGRAVVSCPAACLCASNILSCSKQQLPNVPHSLPSYTALLDLSHNNLSRLRAEWTPTRLTQLHSLLLSHNHLNFISSEAFSPVPNLRYLDLSSNQLRTLDEFLFSDLQVLEVLLLYNNHIMAVDRCAFDDMAQLQKLYLSQNQISRFPLELVKEGAKLPKLTLLDLSSNKLKNLPLPDLQKLPAWIKNGLYLHNNPLNCDCELYQLFSHWQYRQLSSVMDFQEDLYCMNSKKLHNVFNLSFLNCGEYKERAWEAHLGDTLIIKCDTKQQGMTKVWVTPSNERVLDEVTNGTVSVSKDGSLLFQQVQVEDGGVYTCYAMGETFNETLSVELKVHNFTLHGHHDTLNTAYTTLVGCILSVVLVLIYLYLTPCRCWCRGVEKPSSHQGDSLSSSMLSTTPNHDPMAGGDKDDGFDRRVAFLEPAGPGQGQNGKLKPGNTLPVPEATGKGQRRMSDPESVSSVFSDTPIVV

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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Protein Description

AMIGO1 (Adhesion Molecule with Ig-like domain 1) is a member of the immunoglobulin superfamily and plays a significant role in neurodevelopment and cell adhesion processes. Recent studies have highlighted its involvement in various physiological functions, including neuronal differentiation, growth cone dynamics, and synaptogenesis. Abnormal expression or function of AMIGO1 has been linked to several neurodevelopmental disorders and cognitive impairments, making it a potential target for therapeutic interventions. In the context of protein research, the recombinant form of AMIGO1 has been generated to facilitate detailed studies on its structure-function relationship and to explore its interactions with other cellular components. Utilizing advanced techniques such as X-ray crystallography and cryo-electron microscopy, researchers aim to elucidate the molecular mechanisms by which AMIGO1 influences neuronal signaling pathways. Furthermore, the recombinant protein offers opportunities for high-throughput screening of small molecules that could modulate its activity, providing insights that may lead to innovative treatments for related disorders. Overall, the study of AMIGO1 and its recombinant protein has become pivotal in understanding its biological roles and therapeutic potential in neurobiology.

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