Analytical Data
-
Gene name
GATA3
- Application
-
Alternative Names
GATA3；Trans-acting T-cell-specific transcription factor GATA-3
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P23771
-
Expression Region
2-443aa
-
AA Sequence
MASMTGGQQMGRGHHHHHHENLYFQGEVTADQPRWVSHHHPAVLNGQHPD THHPGLSHSYMDAA QYPLPEEVDVLFNIDGQGNHVPPYYGNSVRATVQ RYPPTHHGSQVCRPPLLHGSLPWLDGGKAL GSHHTASPWNLSPFSKTS IHHGSPGPLSVYPPASSSSLSGGHASPHLFTFPPTPPKDVSPDPSL ST PGSAGSARQDEKECLKYQVPLPDSMKLESSHSRGSMTALGGASSSTHHPI TTYPPYVPEYSS GLFPPSSLLGGSPTGFGCKSRPKARSSTGRECVNCG ATSTPLWRRDGTGHYLCNACGLYHKMNG QNRPLIKPKRRLSAARRAGT SCANCQTTTTTLWRRNANGDPVCNACGLYYKLHNINRPLTMKKE GIQT RNRKMSSKSKKCKKVHDSLEDFPKNSSFNPAALSRHMSSLSHISPFSHSS HMLTTPTPMH PPSSLSFGPHHPSSMVTAMG
-
Molecular Weight
51 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GATA3 is a transcription factor that plays a crucial role in various biological processes, including T-cell development, immune response, and mammary gland differentiation. It is a member of the GATA family of zinc-finger proteins and is primarily expressed in T cells and epithelial tissues. Research has indicated that GATA3 is vital for the differentiation of Th2 cells and is implicated in the pathogenesis of several diseases, including allergies and certain types of cancer, particularly breast cancer. The GATA3 protein is frequently studied in the context of its regulatory functions, including its role as an oncogene in tumor progression. The recombinant GATA3 protein is produced through methods such as bacterial or cell-free expression systems, allowing for extensive biophysical characterization and functional assays. Such studies facilitate the understanding of GATA3’s structural properties and its interaction with DNA and other regulatory proteins. By investigating the function and dynamics of GATA3 through recombinant techniques, researchers aim to delineate its precise roles in cellular signaling pathways and to explore its potential as a therapeutic target in conditions like autoimmune diseases and cancer.
