Analytical Data
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基因名
GLI3
- Application
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别名
GLI3；Transcriptional activator GLI3
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P10071
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表达区间
全长
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氨基酸序列
full
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GLI3 is a crucial transcription factor that plays a significant role in the Hedgehog signaling pathway, which is essential for various developmental processes, including limb, brain, and organ formation. Mutations and disruptions in GLI3 function are linked to several human developmental disorders, such as Greig cephalopolysyndactyly syndrome and Pallister-Hall syndrome, highlighting its importance in embryonic development and tissue patterning. Research on GLI3 recombinant proteins has gained prominence, as these proteins can be utilized to study the molecular mechanisms underlying GLI3's activity, its interaction with other proteins, and its functional implications in both normal and pathological states. By producing and characterizing these recombinant proteins in model systems, scientists aim to uncover the precise roles of GLI3 in gene regulation, the impact of mutant versions on cellular processes, and potential therapeutic strategies to address diseases caused by GLI3 dysregulation. This research not only enhances our understanding of developmental biology but also contributes to identifying clinical interventions for GLI3-related conditions, making it a significant focus within the fields of genetics, molecular biology, and medicine.
