Analytical Data
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基因名
EME1
- Application
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别名
EME1；MMS4；Crossover junction endonuclease EME1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q96AY2
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表达区间
1-583aa
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氨基酸序列
MALKKSSPSLDSGDSDSEELPTFAFLKKEPSSTKRRQPEREEKIVVVDISDCEASCPPAPELFSPPVPEIAETVTQTQPVRLLSSESEDEEEFIPLAQRLTCKFLTHKQLSPEDSSSPVKSVLDHQNNEGASCDWKKPFPKIPEVPLHDTPERSAADNKDLILDPCCQLPAYLSTCPGQSSSLAVTKTNSDILPPQKKTKPSQKVQGRGSHGCRQQRQARQKESTLRRQERKNAALVTRMKAQRPEECLKHIIVVLDPVLLQMEGGGQLLGALQTMECRCVIEAQAVPCSVTWRRRAGPSEDREDWVEEPTVLVLLRAEAFVSMIDNGKQGSLDSTMKGKETLQGFVTDITAKTAGKALSLVIVDQEKCFSLELLFFDFLPCTSAQNPPRRGKQGANKQTKKQQQRQPEASIGSMVSRVDAEEALVDLQLHTEAQAQIVQSWKELADFTCAFTKAVAEAPFKKLRDETTFSFCLESDWAGGVKVDLAGRGLALVWRRQIQQLNRVSLEMASAVVNAYPSPQLLVQAYQQCFSDKERQNLLADIQVRRGEGVTSTSRRIGPELSRRIYLQMTTLQPHLSLDSAD
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分子量
80.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EME1, or "essential meiotic endonuclease 1," has emerged as a critical protein in the context of DNA repair and meiotic recombination, which are essential processes for maintaining genomic stability during gametogenesis. Studies indicate that EME1 interacts with key proteins involved in the homologous recombination pathway, playing a crucial role in the processing of DNA double-strand breaks (DSBs). Understanding EME1's structure and function is vital for elucidating the mechanisms of genetic diversity and fertility in various organisms, as improper functioning or regulation of EME1 can lead to chromosomal abnormalities and infertility. Research has shown that EME1 is involved in the formation of Holliday junctions, a key intermediate in the repair process, and its dysregulation may contribute to genomic instability, cancer, and other diseases. The exploration of EME1 through methods such as X-ray crystallography and functional assays has provided insights into its enzymatic activities and interactions with other proteins, paving the way for its potential as a therapeutic target in the context of cancer treatment and reproductive health. Thus, the ongoing investigation into EME1 not only enhances our understanding of fundamental biological processes but also opens avenues for clinical applications in reproductive medicine and cancer therapy.
