Analytical Data
-
Gene name
CRBN
- Application
-
Alternative Names
CRBN；Protein cereblon
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q96SW2
-
Expression Region
1-442aa
-
AA Sequence
MAGEGDQQDAAHNMGNHLPLLPAESEEEDEMEVEDQDSKEAKKPNIINFD TSLPTSHTYLGADMEEFHGRTLHDDDSCQVIPVLPQVMMILIPGQTLPLQ LFHPQEVSMVRNLIQKDRTFAVLAYSNVQEREAQFGTTAEIYAYREEQDF GIEIVKVKAIGRQRFKVLELRTQSDGIQQAKVQILPECVLPSTMSAVQLE SLNKCQIFPSKPVSREDQCSYKWWQKYQKRKFHCANLTSWPRWLYSLYDA ETLMDRIKKQLREWDENLKDDSLPSNPIDFSYRVAACLPIDDVLRIQLLK IGSAIQRLRCELDIMNKCTSLCCKQCQETEITTKNEIFSLSLCGPMAAYV NPHGYVHETLTVYKACNLNLIGRPSTEHSWFPGYAWTVAQCKICASHIGW KFTATKKDMSPQKFWGLTRSALLPTIPDTEDEISPDKVILCL
-
Molecular Weight
77 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CRBN (Cereblon) is a protein that plays a crucial role in the protein degradation system of eukaryotic cells, specifically as part of the CRL4^CRBN E3 ubiquitin ligase complex. This complex is involved in the ubiquitination and subsequent degradation of target proteins, a process vital for maintaining cellular homeostasis and regulating various biological functions, including cell differentiation, proliferation, and apoptosis. The significance of CRBN extends beyond basic cellular functions; mutations and dysregulation of CRBN have been implicated in various diseases, including cancer and neurological disorders. Recent research has highlighted CRBN as a therapeutic target, especially in the context of immunomodulatory drugs used for multiple myeloma, such as lenalidomide and pomalidomide, which leverage CRBN's protein-recruitment capabilities to enhance the degradation of oncogenic proteins. Consequently, understanding the structure and function of recombinant CRBN is pivotal for developing novel therapeutic strategies and improving existing treatments. The study of CRBN not only sheds light on its role in cellular processes but also opens avenues for innovative drug designs that harness the power of ubiquitin-mediated degradation in disease management. Hence, research into CRBN and its recombinant forms continues to be a vibrant area of study within the fields of molecular biology and pharmacology, with the potential to inform future approaches to precision medicine.
