Analytical Data
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基因名
CRBN
- Application
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别名
CRBN；Protein cereblon
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q96SW2
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表达区间
1-442aa
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氨基酸序列
MAGEGDQQDAAHNMGNHLPLLPAESEEEDEMEVEDQDSKEAKKPNIINFD TSLPTSHTYLGADMEEFHGRTLHDDDSCQVIPVLPQVMMILIPGQTLPLQ LFHPQEVSMVRNLIQKDRTFAVLAYSNVQEREAQFGTTAEIYAYREEQDF GIEIVKVKAIGRQRFKVLELRTQSDGIQQAKVQILPECVLPSTMSAVQLE SLNKCQIFPSKPVSREDQCSYKWWQKYQKRKFHCANLTSWPRWLYSLYDA ETLMDRIKKQLREWDENLKDDSLPSNPIDFSYRVAACLPIDDVLRIQLLK IGSAIQRLRCELDIMNKCTSLCCKQCQETEITTKNEIFSLSLCGPMAAYV NPHGYVHETLTVYKACNLNLIGRPSTEHSWFPGYAWTVAQCKICASHIGW KFTATKKDMSPQKFWGLTRSALLPTIPDTEDEISPDKVILCL
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分子量
77 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CRBN (Cereblon) is a protein that plays a crucial role in the protein degradation system of eukaryotic cells, specifically as part of the CRL4^CRBN E3 ubiquitin ligase complex. This complex is involved in the ubiquitination and subsequent degradation of target proteins, a process vital for maintaining cellular homeostasis and regulating various biological functions, including cell differentiation, proliferation, and apoptosis. The significance of CRBN extends beyond basic cellular functions; mutations and dysregulation of CRBN have been implicated in various diseases, including cancer and neurological disorders. Recent research has highlighted CRBN as a therapeutic target, especially in the context of immunomodulatory drugs used for multiple myeloma, such as lenalidomide and pomalidomide, which leverage CRBN's protein-recruitment capabilities to enhance the degradation of oncogenic proteins. Consequently, understanding the structure and function of recombinant CRBN is pivotal for developing novel therapeutic strategies and improving existing treatments. The study of CRBN not only sheds light on its role in cellular processes but also opens avenues for innovative drug designs that harness the power of ubiquitin-mediated degradation in disease management. Hence, research into CRBN and its recombinant forms continues to be a vibrant area of study within the fields of molecular biology and pharmacology, with the potential to inform future approaches to precision medicine.
