Analytical Data
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基因名
MLKL
- Application
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别名
MLKL；Mixed lineage kinase domain-like Protein
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8NB16
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表达区间
1-471aa
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氨基酸序列
MENLKHIITLGQVIHKRCEEMKYCKKQCRRLGHRVLGLIKPLEMLQDQGK RSVPSEKLTTAMNRFKAALEEANGEIEKFSNRSNICRFLTASQDKILFKD VNRKLSDVWKELSLLLQVEQRMPVSPISQGASWAQEDQQDADEDRRAFQM LRRDNEKIEASLRRLEINMKEIKETLRQYLPPKCMQEIPQEQIKEIKKEQ LSGSPWILLRENEVSTLYKGEYHRAPVAIKVFKKLQAGSIAIVRQTFNKE IKTMKKFESPNILRIFGICIDETVTPPQFSIVMEYCELGTLRELLDREKD LTLGKRMVLVLGAARGLYRLHHSEAPELHGKIRSSNFLVTQGYQVKLAGF ELRKTQTSMSLGTTREKTDRVKSTAYLSPQELEDVFYQYDVKSEIYSFGI VLWEIATGDIPFQGCNSEKIRKLVAVKRQQEPLGEDCPSELREIIDECRA HDPSVRPSVDEILKKLSTFSK
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分子量
59 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MLKL (Mixed Lineage Kinase Domain-Like Protein) is a crucial effector in the pathway of programmed necrosis, particularly in the context of necroptosis, a form of regulated cell death distinct from apoptosis. Initially identified as a key player in the TNF receptor signaling cascade, MLKL is activated through phosphorylation by the kinases RIPK1 and RIPK3, leading to its oligomerization and subsequent translocation to the plasma membrane. This process disrupts cellular integrity and triggers inflammation, marking its role in various pathological conditions, including neurodegenerative diseases, inflammatory disorders, and cancer. Given its significance in necroptosis, researchers have focused on elucidating the molecular mechanisms governing MLKL function and the implications of its activity in disease contexts. Advances in structural biology have facilitated the characterization of MLKL's oligomeric states and provided insights into its interaction with lipid membranes, which are critical for understanding its role in cell death and inflammation. The development of MLKL inhibitors has emerged as a promising therapeutic strategy to modulate necroptosis, potentially offering avenues for treating diseases where this process is dysregulated. Hence, the study of MLKL, particularly through recombinant protein techniques, is pivotal for both basic biological research and the development of innovative treatments targeting necroptosis-related pathologies.
