Analytical Data
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基因名
CYBA
- Application
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别名
CYBACytochrome b-245 light chain; Cytochrome b(558) alpha chain; Cytochrome b558 subunit alpha; Neutrophil cytochrome b 22 kDa polypeptide; Superoxide-generating NADPH oxidase light chain subunit; p22 phagocyte B-cytochrome; p22-phox; p22phox
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P13498
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表达区间
2-195aa
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氨基酸序列
GQIEWAMWA NEQALASGLI LITGGIVATA GRFTQWYFGA YSIVAGVFVC LLEYPRGKRK KGSTMERWGQ KYMTAVVKLF GPFTRNYYVR AVLHLLLSVP AGFLLATILG TACLAIASGI YLLAAVRGEQ WTPIEPKPRE RPQIGGTIKQ PPSNPPPRPP AEARKKPSEE EAAVAAGGPP GGPQVNPIPV TDEVV
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分子量
21 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CYBA, also known as cytochrome b-245 alpha chain, plays a crucial role in the functioning of the NADPH oxidase complex, which is essential for generating reactive oxygen species (ROS) involved in various physiological processes and immune responses. Research into the recombinant form of CYBA protein has gained traction due to its importance in understanding oxidative stress-related diseases, such as chronic inflammation, cardiovascular disorders, and neurodegenerative diseases. The recombinant CYBA protein allows for detailed studies of its structure-function relationships and interaction with other subunits of the NADPH oxidase complex. Furthermore, the expression and purification of CYBA in suitable systems enable the investigation of its enzymatic mechanisms and the modulation of ROS production. Advances in recombinant technology have facilitated the large-scale production of CYBA, paving the way for drug discovery and the development of therapeutic interventions targeting oxidative stress pathways. Investigating the properties and functions of CYBA through recombinant protein studies could ultimately lead to novel strategies for managing diseases associated with dysregulated ROS levels, highlighting the significance of this research in biomedical science.
