Analytical Data
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基因名
RAD50
- Application
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别名
RAD50；DNA repair Protein RAD50
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q92878
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表达区间
1-235aa
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氨基酸序列
MSRIEKMSILGVRSFGIEDKDKQIITFFSPLTILVGPNGAGKTTIIECLKYICTGDFPPGTKGNTFVHDPKVAQETDVRAQIRLQFRDVNGELIAVQRSMVCTQKSKKTEFKTLEGVITRTKHGEKVSLSSKCAEIDREMISSLGVSKAVLNNVIFCHQEDSNWPLSEGKALKQKFDEIFSATRYIKALETLRQVRQTQGQKVKEYQMELKYLKQYKEKACEIRDQITSKEAQLT
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分子量
34.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RAD50 is a crucial component of the MRE11-RAD50-NBS1 (MRN) complex, which plays a significant role in the cellular response to DNA double-strand breaks (DSBs), a type of severe DNA damage that can lead to genomic instability and cancer if not properly repaired. The MRN complex is pivotal for sensing DNA damage, facilitating its repair through homologous recombination, and maintaining genomic integrity. RAD50, in particular, is essential for the recruitment of repair proteins to sites of DSBs and is involved in the activation of ATM (Ataxia Telangiectasia Mutated) kinase, a key regulator of the DNA damage response pathway. Recent studies have highlighted the importance of RAD50 not only in normal cellular function but also in the context of various cancers, where mutations or dysregulation of RAD50 may contribute to tumorigenesis and resistance to conventional therapies. Understanding RAD50's structure and function, as well as its interactions within the MRN complex and with other cellular factors, has significant implications for developing targeted cancer therapies and enhancing our understanding of the molecular mechanisms underlying DNA repair processes. This research aims to elucidate RAD50's role in maintaining genomic stability and its potential as a therapeutic target in cancer treatment.
