Analytical Data
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Gene name
SLC28A1
- Application
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Alternative Names
CNT 1; CNT1; Concentrative nucleoside transporter 1; hCNT1; Na(+)/nucleoside cotransporter 1; S28A1_HUMAN; SLC28A1; Sodium-coupled nucleoside transporter 1; Sodium/nucleoside cotransporter 1; solute carrier family 28 concentrative nucleoside transporter member 1; Solute carrier family 28 member 1
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O00337
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AA Sequence
MENDPSRRRESISLTPVAKGLENMGADFLESLEEGQLPRSDLSPAEIRSSWSEAAPKPFSRWRNLQPALRARSFCREHMQLFRWIGTGLLCTGLSAFLLVACLLDFQRALALFVLTCVVLTFLGHRLLKRLLGPKLRRFLKPQGHPRLLLWFKRGLALAAFLGLVLWLSLDTSQRPEQLVSFAGICVFVALLFACSKHHCAVSWRAVSWGLGLQFVLGLLVIRTEPGFIAFEWLGEQIRIFLSYTKAGSSFVFGEALVKDVFAFQVLPIIVFFSCVISVLYHVGLMQWVILKIAWLMQVTMGTTATETLSVAGNIFVSQTEAPLLIRPYLADMTLSEVHVVMTGGYATIAGSLLGAYISFGIDATSLIAASVMAAPCALALSKLVYPEVEESKFRREEGVKLTYGDAQNLIEAASTGAAISVKVVANIAANLIAFLAVLDFINAALSWLGDMVDIQGLSFQLICSYILRPVAFLMGVAWEDCPVVAELLGIKLFLNEFVAYQDLSKYKQCRLAGAEEWVGDRKQWISVRAEVLTTFALCGFANFSSIGIMLGGLTSMVPQRKSDFSQIVLRALFTGACVSLVNACMAGILYMPRGAEVDCMSLLNTTLSSSSFEIYQCCREAFQSVNPEFSPEALDNCCRFYNHTICAQ
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Molecular Weight
71.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SLC28A1, a member of the solute carrier family, is a high-affinity nucleoside transporter predominantly involved in the cellular uptake of nucleosides, which are essential for various physiological processes including DNA and RNA synthesis. Its role in transporting purine and pyrimidine nucleosides makes it crucial for maintaining intracellular nucleotide pools and supporting cellular proliferation, particularly in rapidly dividing cells. Dysregulation of SLC28A1 has been implicated in various conditions, including cancer and viral infections, where altered nucleoside metabolism can affect tumor growth and viral replication. Research into the recombinant protein of SLC28A1 aims to elucidate its structural and functional properties, providing insights into its transport mechanism and interaction with potential ligands. Understanding these properties is vital for the development of targeted therapies that exploit SLC28A1's role in nucleoside transport, potentially leading to innovative treatments for cancer and viral diseases. Furthermore, characterizing the recombinant form of SLC28A1 can facilitate the screening of novel inhibitors or activators, which could be integral in refining therapeutic strategies and enhancing our grasp of nucleoside dynamics in various pathophysiological contexts. Consequently, studies on SLC28A1 not only contribute to the foundation of molecular transport biology but also pave the way for future clinical applications.
