Analytical Data
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Gene name
NUP50
- Application
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Alternative Names
NUP50；NPAP60L；Nuclear pore complex Protein Nup50
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9UKX7
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Expression Region
1-468aa
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AA Sequence
MAKRNAEKEL TDRNWDQEDE AEEVGTFSMA SEEVLKNRAI KKAKRRNVGF ESDTGGAFKG FKGLVVPSGG GRFSGFGSGA GGKPLEGLSN GNNITSAPPF ASAKAAADPK VAFGSLAANG PTTLVDKVSN PKTNGDSQQP SSSGLASSKA CVGNAYHKQL AALNCSVRDW IVKHVNTNPL CDLTPIFKDY EKYLANIEQQ HGNSGRNSES ESNKVAAETQ SPSLFGSTKL QQESTFLFHG NKTEDTPDKK MEVASEKKTD PSSLGATSAS FNFGKKVDSS VLGSLSSVPL TGFSFSPGNS SLFGKDTTQS KPVSSPFPTK PLEGQAEGDS GECKGGDEEE NDEPPKVVVT EVKEEDAFYS KKCKLFYKKD NEFKEKGIGT LHLKPTANQK TQLLVRADTN LGNILLNVLI PPNMPCTRTG KNNVLIVCVP NPPIDEKNAT MPVTMLIRVK TSEDADELHK ILLEKKDA
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NUP50, a nucleocytoplasmic shuttling protein, plays a critical role in the transportation of proteins between the nucleus and cytoplasm, primarily by promoting the nuclear import of various factors. It is a member of the nucleoporins, which constitute the nuclear pore complex, facilitating the bidirectional transport of macromolecules. Recent studies have highlighted the importance of NUP50 in various biological processes, including gene expression regulation, cell cycle progression, and responses to cellular stress. Additionally, NUP50 has been implicated in several diseases, including cancer, where it may influence oncogenic signaling pathways and tumor progression. The recombinant expression and purification of NUP50 are essential for elucidating its structure-function relationships and understanding its interactions with other nuclear transport components. By generating NUP50 as a recombinant protein, researchers can perform biochemical assays, structural studies, and functional analyses to uncover its mechanisms of action, potential post-translational modifications, and role in the pathophysiology of diseases. The exploration of NUP50 through recombinant techniques not only contributes to the fundamental understanding of nucleocytoplasmic transport but also opens avenues for therapeutic interventions targeting malfunctioning transport pathways in various conditions.
